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HPLC Analysis of Triacylglycerols in Soybean Oil on Discovery® HS C18 (Acetone-Acetonitrile Mobile Phase, ELSD Detection)

HPLC Analysis of Triacylglycerols in Soybean Oil on Discovery® HS C18 (Acetone-Acetonitrile Mobile Phase, ELSD Detection) application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

Discovery® HS C18 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

standard

Product No.
Description
Pricing

Glyceryl trioleate

≥99%

Glyceryl trilinoleate

≥98% (TLC), liquid

Glyceryl trilinolenate

≥97% (TLC), liquid

Soybean oil

analytical standard

CONDITIONS

column

Discovery HS C18, 25 cm x 4.6 mm I.D., 5 μm particles (568523-U)

mobile phase

[A] acetone; [B] acetonitrile; (60:40, A:B)

flow rate

1.5 mL/min

pressure

914 psi (63 bar)

column temp.

30 °C

detector

ELSD

injection

5 μL

sample

5% soybean oil in acetone (w/v)

Description

Analysis Note

Soybean oil was analyzed as 5% diluted sample in acetone (w/v)

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany