LC/MS Analysis of Cannabinoids on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

[A] 10 mM ammonium acetate (pH 5.0 with acetic acid): [B] acetonitrile; (20:80, A:B)

flow rate

1 mL/min, split to the MS

column temp.

35 °C

detector

MS ESI (+), SIR Mode

injection

5 μL

sample

1 μg/mL in water:acetonitrile (1:1)