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LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5

LC/MS/MS Analysis of Vitamins B1 and B6 on Ascentis® Express OH5 application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53757-U

Ascentis® Express OH5, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

standard

Product No.
Description
Pricing

47858

Thiamine Hydrochloride (B1)

analytical standard

47862

Pyridoxine Hydrochloride (B6)

analytical standard

CONDITIONS

column

Ascentis Express  OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U)

mobile phase

[A] water with 0.1% formic acid/10 mM ammonium formate; [B] acetonitrile with 0.1% formic acid/10 mM ammonium formate; (A:B, 15:85)

flow rate

0.2 mL/min

pressure

1150 psi (79 bar)

column temp.

30 °C

detector

MS, ESI(+), MRM mode

injection

1 μL

sample

10 ng/mL in mobile phase

Description

Analysis Note

The present work establishes an HILIC LC/MS/MS method for analysis of water soluble vitamins B1 and B6 on an Ascentis® Express OH5 column. LC/MS grade solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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