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UHPLC Analysis of Nucleosides on Purospher® STAR RP-18 endcapped

UHPLC Analysis of Nucleosides on Purospher® STAR RP-18 endcapped application for HPLC

Materials

Standard

Product No.
Description
Pricing

5-Methylcytidine

≥99%

7-Methylguanosine

≥90%

Uridine

≥99%

Cytidine

BioReagent, suitable for cell culture, powder, ≥99%

Inosine

≥99% (HPLC)

Guanosine

≥98%

Nucleosides Test Mix

in 1% sodium formate (varied conc.), analytical standard

Adenosine

Pharmaceutical Secondary Standard; Certified Reference Material

Disodium Guanylate

Pharmaceutical Secondary Standard; Certified Reference Material

Uridine

Pharmaceutical Secondary Standard; Certified Reference Material

Guanosine

Pharmaceutical Secondary Standard; Certified Reference Material

5′-Adenylic Acid

Pharmaceutical Secondary Standard; Certified Reference Material

Zidovudine Related Compound D

pharmaceutical secondary standard

Cytidine

certified reference material, pharmaceutical secondary standard

CONDITIONS

column

Hibar Purospher® STAR RP-18 endcapped, 150 x 4.6 mm, 3 μm (1.50470.0001)

mobile phase

[A] 30 mM ammonium phosphate, pH 5.3 with ammonium hydroxide in water; [B] Methanol; 97.5 [A] / 2.5 [B]

flow rate

1.0 mL/min

pressure

3060 psi 211 Bar

column temp.

25 °C

detector

UV 254

injection

5 μL

sample

5 μg/mL of 12 nucleosides in 98:2 30mM ammonium phosphate pH 5.3 with ammonium hydroxide in water: methanol

Description

Analysis Note

Nucleosides are structural subunits of nucleic acids, the macromolecules that convey genetic information in living cells. They contain a molecule of sugar (pentose) bonded to a nitrogen-containing organic ring compound. The nitrogen-containing compound is either a pyrimidine (cytosine, thymine, or uracil) or a purine (adenine or guanine). Nucleosides, derivatives and / or analogues can be used in many different industries and applications as in agrochemistry (herbicides, fungicides and insecticides), therapeutic drugs including a range of antiviral (HIV, hepatitis C virus and herpes simplex) and even antibiotics.

Legal Information

Purospher is a registered trademark of Merck KGaA, Darmstadt, Germany