USP Analysis of Actos (Pioglitazone) using Ascentis^® Express C18

column

Ascentis^® Express C18, 15 cm x 4.6 mm, 5 μm particles (50537-U)

mobile phase

[A] 100 mM ammonium acetate; [B] acetonitrile; [C] acetic acid; (55:45:2, A:B:C)

flow rate

0.7 mL/min

pressure

1160 psi (80 bar)

column temp.

25 °C

detector

UV, 269 nm

injection

20 μL

sample

pioglitazone 50 μg/mL, benzophenone 13 μg/mL in mobile phase