Skip to Content
MilliporeSigma

US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18

US EPA Method 632: HPLC Analysis of Pesticides on Ascentis® C18 application for HPLC

Materials

Analytical column

Product No.
Description
Pricing

Ascentis® C18 HPLC Column

3 μm particle size, L × I.D. 15 cm × 4.6 mm

Ascentis® C18 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

Ascentis® C8 HPLC Column

3 μm particle size, L × I.D. 15 cm × 4.6 mm

Ascentis® C8 HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

Ascentis® Express C18, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

Ascentis® Express C8, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

accessory

Product No.
Description
Pricing

Wheaton wide-mouth bottle

amber soda-lime glass bottle, capacity 1000 mL, white polypropylene cap, (PTFE faced LDPE liner), case of 12 ea

Coupler for Legacy Guard Column Holder

PEEK, I.D. × O.D. × Overall L 0.010 in. × 1/16 in. × 1 in.

Supelguard Guard Cartridge Holder

Stand-alone, for use with Supelguard cartridges (2 cm L. x 2.1 to 4.0 mm I.D.)

guard cartridge

Product No.
Description
Pricing

Ascentis® C18 Supelguard Guard Cartridge

5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea

Ascentis® C18 Supelguard Guard Cartridge

3 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea

Ascentis® C8 Supelguard Guard Cartridge

5 μm particle size, L × I.D. 2 cm × 4 mm, pkg of 2 ea

CONDITIONS

column

Ascentis C18, 25 cm x 4.6 mm I.D., 5 μm particles (581325-U)

mobile phase

[A] water; [B] acetonitrile

gradient

10 to 50% B in 13 min; to 60% B in 10 min; to 90% B in 6 min; held for 5 min

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 210 nm

injection

10 μL

sample

as indicated in 16% acetonitrile in water

Description

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany