UHPLC-MS Analysis of Basic Drugs and Metabolites with Titan C18

column

Titan C18 10 cm x 2.1 mm, 1.9 μm particles (577124-U)

mobile phase

[A] Water; [B] acetonitrile, both with 0.1% formic acid

gradient

35%B Isocratic

flow rate

0.4 mL/min

pressure

484 psi (7120)

column temp.

25 °C

detector

MS, ESI(+), MRM

injection

1 μL

sample

50 ng/mL in 50% methanol