UHPLC Analysis of Curcumins on Ascentis^® Express RP-Amide

mobile phase

[A] water with 0.1% formic acid; [B] acetonitrile with 0.1% formic acid;

gradient

40% B held for 1 min; 40 to 100% B in (6 min); 100% B held for 5 min

flow rate

1.0 mL/min

pressure

1270 psi (88 bar)

column temp.

35 °C

detector

UV, 250 nm

injection

5 μL

sample

5 curcumins 50μg/mL in 50:50, water:methanol

column

Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 5 μm particles (50774-U)