ZooMAb® Recombinant Antibody Reconstitution Protocol
Ready to reconstitute your ZooMAb® recombinant antibody? Follow along with the short antibody reconstitution protocol below to see how easy it is.
How To Reconstitute ZooMAb® Recombinant Antibodies
ZooMAb® recombinant antibodies represent a new generation of monoclonal antibodies that are based on sequence-defined recombinant expression. These antibodies are specifically engineered using our proprietary recombinant antibody technologies to provide state-of-the-art consistency and application performance. They are also designed with user-friendly formulation, handling, and storage features plus validation in multiple applications.
ZooMAb® antibodies are provided as a lyophilized product. The following short protocol is recommended for antibody reconstitution.
![A single vial of ZooMAb® recombinant antibody at room temperature. A single vial of ZooMAb® recombinant antibody at room temperature.](/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/protein-biology/immunohistochemistry/zoomab-antibody-vial/zoomab-antibody-vial.jpg)
![MyFuge™ mini centrifuge with open lid that can be used to spin down ZooMAb® antibody vial. MyFuge™ mini centrifuge with open lid that can be used to spin down ZooMAb® antibody vial.](/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/protein-biology/immunohistochemistry/myfuge-mini-centrifuge/myfuge-mini-centrifuge.jpg)
Spin
Microfuge briefly (~5 seconds) to spin down the material to the bottom of the vial.
![Gloved hand holding pipette to add purified water or PBS to antibody vial. Gloved hand holding pipette to add purified water or PBS to antibody vial.](/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/protein-biology/immunohistochemistry/pipetting/pipetting.jpg)
Add Liquid
Add 25 µL of filtered laboratory grade water or phosphate buffered saline (PBS) to vial.
![MilliSentials™ vortexer that can be used to vortex the antibody vial. MilliSentials™ vortexer that can be used to vortex the antibody vial.](/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/protein-biology/immunohistochemistry/millisentials-vortexer/millisentials-vortexer.jpg)
Vortex
Vortex the vial for 20-30 seconds.
![Stopwatch with 5 minutes on it. Stopwatch with 5 minutes on it.](/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/protein-biology/immunohistochemistry/stopwatch/stopwatch.jpg)
Wait
Wait for 5-10 minutes.
![MyFuge™ mini centrifuge with open lid that can be used to spin down solubilized liquid in the ZooMAb® antibody vial. MyFuge™ mini centrifuge with open lid that can be used to spin down solubilized liquid in the ZooMAb® antibody vial.](/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/protein-biology/immunohistochemistry/myfuge-mini-centrifuge/myfuge-mini-centrifuge.jpg)
Spin
Microfuge vial briefly (~5 seconds) for complete solubilization and to spin down the liquid to the bottom of the vial.
![A single vial of ZooMAb® recombinant antibody that is ready to use. A single vial of ZooMAb® recombinant antibody that is ready to use.](/deepweb/assets/sigmaaldrich/marketing/global/images/technical-documents/protocols/protein-biology/immunohistochemistry/zoomab-antibody-vial/zoomab-antibody-vial.jpg)
Ready for Use
The reconstituted antibody is now ready for use. It can be stored at 2-8 ⁰C for short term or at -20 ⁰C for long-term. Avoid repeated freeze-thaw cycles.
Related Products
Browse our highlighted antibodies below, or search all of them on our ZooMAb® antibodies page.
For Research Use Only. Not For Use In Diagnostic Procedures.
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