Ascentis Express HPLC columns, based on Fused-Core particle technology, provide more than twice the speed and efficiency of traditional columns at half the backpressure of sub-2-µm columns. This performance enhancement is applicable to all HPLC instruments (in addition to UHPLC systems).
Why do columns based on Fused-Core technology offer superior performance? The Fused-Core particle has three characteristic features over the traditional porous particle, resulting in a number of performance benefits - especially for Fast HPLC and UHPLC applications.
Features of Fused-Core particles over traditional porous particles
For an in-depth explanation, with practical examples that demonstrate the performance benefits of Fused-Core technology, watch the presentation linked on this page.
Traditional Porous Particles
The innovative manufacturing process for Fused-Core particles produces a very narrow particle size distribution. A narrow particle size distribution allows for the use of large porosity frits that resist clogging, resulting in a more rugged column.
Traditional porous particles are not manufactured in a way to yield extremely narrow particle size distributions.
The “A” term in the van Deemter equation accounts for the effects of inhomogeneities in the packed bed of an HPLC column. Narrow particle size distributions form a more consistent packed bed and a consistent path length, minimizing analyte diffusion through the column. This eddy diffusion is effectively independent of mobile phase velocity.
The short diffusion path of the Fused-Core particle yields sharper peaks than traditional porous particle columns. The minimized resistance to mass transfer, the “C” term in the van Deemter equation, of the Fused-Core particle provides sharper peaks than traditional porous particles. The short diffusion path also permits the use of higher flow rates without peak broadening.
Fused-Core - Advanced Materials Technology, Inc.
To continue reading please sign in or create an account.Don't Have An Account?