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column	Ascentis^® Express RP-Amide, 5 cm x 3 mm I.D., 2.7 µm particles (53916-U)
column temp.	55 °C
mobile phase	[A] 10 mM ammonium phosphate monobasic, pH 2.5 with phosphoric acid; [B] ethanol; (75:25, A:B)
flow rate	0.4 mL/min
pressure	138 bar (2000 psi) column, 248 bar (3600 psi) total
sample	50 µg/mL in mobile phase
injection	1 µL
detector	UV, 215 nm

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Categories	Analytical Chromatography, Organic Acids
Featured Industry	Cosmetics, Personal Care, and Cleaning Products
Legal Information	Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability	application for HPLC

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HPLC Analysis of Benzoic Acid and Sorbic Acid on Ascentis® Express RP-Amide ("Green" Mobile Phase Conditions)

Conditions

Description

HPLC Analysis of Benzoic Acid and Sorbic Acid on Ascentis^® Express RP-Amide ("Green" Mobile Phase Conditions)