These products are available in the US, Canada, and select European countries.
Adipose tissue is essential for energy storage and metabolic homeostasis of the body. Human White Preadipocytes (HWP) are self-renewing progenitors of mature differentiated adipocytes and can be found as a constitutive subpopulation in adipose tissue in adults.
PromoCell® Human White Preadipocytes are produced from normal human subcutaneous and visceral adipose tissue from varied anatomical sites (lot-specific source information is available on request). Differentiation of HWP into mature adipocytes can be performed using the PromoCell® Preadipocyte Differentiation Media system. Shortly after isolation, all Human White Preadipocytes are cryopreserved at passage 2 (P2) using proprietary serum-free freezing medium Cryo-SFM. Each cryovial contains at least 500,000 viable cells after thawing. Human White Preadipocytes are also available as proliferating cell cultures that are generated from cryopreserved cells that have been thawed and cultured for three days.
A range of culture media have been formulated for the undifferentiated expansion of human preadipocytes, differentiation of preadipocytes into mature adipocytes, and in vitro cultivation of human mature adipocytes. These media are optimized for growing primary human cells, but may also be used to cultivate murine preadipocytes/adipocytes.
All Preadipocyte/Adipocyte Media are available in two formats: Medium (ready-to-use) and Medium Kit. The Medium (ready-to-use) option consists of a 500 mL bottle of Basal Medium and one vial of SupplementMix. The Medium Kit consists of a 500 mL bottle of Basal Medium and the SupplementPack (a set of individual vials with pre-measured supplements) to provide the user complete control over the media formulation. Adding the SupplementMix or the SupplementPack to the Basal Medium constitutes the complete medium. The Basal Medium (with or without phenol red), SupplementMix, and SupplementPack are also available for individual purchase.
The Preadipocyte Growth Medium contains all the growth factors and supplements necessary for the optimal growth of undifferentiated human preadipocytes. The Preadipocyte Differentiation Medium is a serum-free medium containing all supplements for effectively inducing the differentiation of preadipocytes into mature adipocytes, including ciglitazone. The Adipocyte Nutrition Medium is a low-serum medium containing all the growth factors and supplements necessary for the maintenance of mature adipocytes. These media do not contain antibiotics or antimycotics and are formulated for use in an incubator with an atmosphere of 5% CO2.
Note: Light flocculation may be seen upon thawing the supplements containing ECGS. This does not affect reagent activity. Optionally, the precipitate can be removed by centrifugation under sterile conditions.
Human White Preadipocytes (HWP) Quality Control
Rigid quality control tests are performed for each lot of Human White Preadipocytes.
All lots of Preadipocyte/Adipocyte Media are subjected to comprehensive quality control tests using primary human white preadipocytes. Each lot is checked for growth-promoting activity, adherence promotion, and typical morphology of the tested preadipocytes. In addition, the Preadipocyte Differentiation Medium/Adipocyte Nutrition Medium are tested for the capacity to induce the terminal differentiation of preadipocytes into mature adipocytes. Approved in-house lots of Preadipocyte/Adipocyte Media are used as reference. All lots of media are rigorously tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).
Intended Use for Primary Preadipocyte/Adipocyte Culture Systems
These products are for in vitro use only and not for diagnostic or therapeutic procedures. For safety precautions please see appropriate MSDS.
Note: Due to their low serum content or the absence of serum, PromoCell media are not suitable for trypsin neutralization (when subculturing the cells, for example). Instead we recommend using our DetachKit (C-41200, C-41210, C-41220), which contains HEPES BSS, Trypsin/EDTA and Trypsin Neutralizing Solution.
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