B4554
BES
BioPerformance Certified, suitable for cell culture, ≥99.0%
Synonym(s):
N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
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About This Item
Empirical Formula (Hill Notation):
C6H15NO5S
CAS Number:
Molecular Weight:
213.25
Beilstein:
1781572
EC Number:
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25
Recommended Products
grade
BioPerformance Certified
Assay
≥99.0%
form
crystalline
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin and bioburden, tested
pH
2.5-5.0 (25 °C, 213.3 g/L)
useful pH range
6.4-7.8
pKa (25 °C)
7.1
solubility
water: 0.5 g/mL, clear, colorless
application(s)
diagnostic assay manufacturing
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General description
N-N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) is a zwitterionic sodium sulphate buffer used in biochemistry and molecular biology research. BES buffer used as a secondary pH standard can help maintain a stable pH range in solutions, particularly in the range of 6.2-7.6 pH range of 6.6 to 7.4. It is used in a study to evaluate that buffers minimize the action of interfering factors in the bacterial endotoxins test. The application of BES as a buffer solution for calcium phosphate-mediated transfection of eukaryotic cells with plasmid DNA has also been studied.
Application
BES buffer has been used:
- as a supplement in opti-minimal essential medium (MEM) culture media to culture thymocytes for In vitro analysis of thymocyte apoptosis
- as a component of borate buffer saline (BBS) for adeno-associated virus (AAV) vector production, extraction, and purification
- as a component of diluent one for semen collection and cryopreservation
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Effects of amino acid sequence, buffers, and ionic strength on the rate and mechanism of deamidation of asparagine residues in small peptides
Tyler-Cross R and Schirch V
The Journal of Biological Chemistry, 266(33) , 22549-22556 (1991)
Calcium-phosphate-mediated Transfection of Eukaryotic Cells with Plasmid DNAs
Sambrook J and Russell D W
CSH Protocols, 2006(1) (2006)
Cryopreservation of Korean Oge chicken semen using N-methylacetamide
Hong Jo L, et al.
Cryo letters, 33(6), 427-434 (2012)
Standard buffer of N, N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (Bes) for use in the physiological pH range 6.6 to 7.4
Roy R N, et al.
Analytical Chemistry, 47(8) (1975)
Saline and buffers minimize the action of interfering factors in the bacterial endotoxins test
Fujita Y, et al.
Analytical Biochemistry, 409(1), 46-53 (2011)
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