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B9879

Sigma-Aldrich

BES

≥99.0% (titration)

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Synonym(s):
2-(bis(2-hydroxyethyl)amino)ethane sulfonic acid, n,n-bis[2-hydroxyethyl]-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
Empirical Formula (Hill Notation):
C6H15NO5S
CAS Number:
Molecular Weight:
213.25
Beilstein:
1781572
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Assay

≥99.0% (titration)

form

crystalline

pH

2.5-5.0 (25 °C, 213.3 g/L)

useful pH range

6.4-7.8

pKa (25 °C)

7.1

solubility

water: 0.5 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

storage temp.

room temp

SMILES string

OCCN(CCO)CCS(O)(=O)=O

InChI

1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)

InChI key

AJTVSSFTXWNIRG-UHFFFAOYSA-N

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This Item
3913341.15228SAB1403877
BES ≥99.0% (titration)

Sigma-Aldrich

B9879

BES

BES ULTROL® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

Millipore

391334

BES

form

crystalline

form

solid

form

solid

form

buffered aqueous solution

pH

2.5-5.0 (25 °C, 213.3 g/L)

pH

-

pH

-

pH

-

solubility

water: 0.5 g/mL, clear, colorless

solubility

water: 1 mg/mL

solubility

-

solubility

-

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

-

application(s)

-

storage temp.

room temp

storage temp.

15-25°C

storage temp.

2-30°C

storage temp.

−20°C

General description

N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, known as BES, is a zwitterionic buffer and a valuable secondary standard in biochemical research. BES, a sulfonic acid-containing cross-linking agent, induces cross-linking between sulfonated polyimide chains in sulfonated polyimide membranes. The useful pH range for BES buffer is 6.4 to 7.8. It is one of the "Good" buffers developed in the 1960s to provide buffering capacity in the pH range of 6.15 to 8.35, making it widely applicable in biochemical studies. BES buffer is suitable for use in systems where the formation of a calcium phosphate-DNA complex is desirable (Absorbance: < 0.05 at 260 nm, 100 mM). Further, BES buffer can be employed in the study of molecular biology, biological buffers, molecular biology reagents, and zwitterionic compounds.

Application

BES may be employed as binding buffer in modified Eagle′s medium during the binding assay of human melanoma cells. It may be used as biobuffer to investigate the aqueous medium self-assembly of heterometallic CuII/Li 3D coordination polymers.
Further, BES has found application in transfection studies, both in the preparation of reagents for transfection and in signaling assays related to Neural Melanocortin Receptors.

Features and Benefits

  • Suitable for Cell Culture media and as a Buffering solution in Biochemical and Molecular Biology Research
  • Effective Buffering from pH 2.5-5.0 (25 °C, 213.3 g/L) with a pKa of 7.1 (25 °C)
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

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Water sorption, proton conduction, and methanol permeation properties of sulfonated polyimide membranes cross-linked with N, N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (BES).
Lee CH, et al.
Macromolecules, 39(2), 755-764 (2006)
Second-stage dissociation of N,N-bis (2-hydroxyethyl)-2-aminoethanesulfonic acid (?bes?) in water and in 50 mass per cent methanol+ water from 278.15 to 328.15 K.
Roy RN, et al.
The Journal of Chemical Thermodynamics, 9(4), 325-332 (1977)
Marina V Kirillova et al.
Inorganic chemistry, 51(9), 5224-5234 (2012-04-10)
The facile aqueous medium reactions of copper(II) nitrate with BES biobuffer [(HOCH(2)CH(2))(2)N(CH(2)CH(2)SO(3)H), hereinafter referred as H(3)bes] in the presence of various benzenecarboxylic acids [benzoic (Hba), 3-hydroxybenzoic (Hhba), and 3,5-dihydroxybenzoic (Hdhba) acid] and lithium hydroxide gave rise to the self-assembly generation
R N Fabricant et al.
Proceedings of the National Academy of Sciences of the United States of America, 74(2), 565-569 (1977-02-01)
Purified mouse nerve growth factor (NGF)radiolabeled with 125I was tested for its ability to bind to a variety of different cultured cells. NGF binds readily to human and hamster melanoma cells but does not bind to many other cell lines.
Ya-Xiong Tao et al.
Methods in enzymology, 484, 267-279 (2010-11-03)
The two neural melanocortin receptors (MCRs), melanocortin-3 and -4 receptors (MC3R and MC4R), are G protein-coupled receptors expressed primarily in the brain that regulate different aspects of energy homeostasis. The MCRs are unique in having endogenous antagonists, agouti and agouti-related

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