123072
Luminol
97%
Synonym(s):
3-Aminophthalhydrazide, 5-Amino-2,3-dihydro-1,4-phthalazinedione
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About This Item
Empirical Formula (Hill Notation):
C8H7N3O2
CAS Number:
Molecular Weight:
177.16
Beilstein:
383929
EC Number:
MDL number:
UNSPSC Code:
12352103
PubChem Substance ID:
NACRES:
NA.23
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Quality Level
Assay
97%
mp
>300 °C (lit.)
SMILES string
Nc1cccc2C(=O)NNC(=O)c12
InChI
1S/C8H7N3O2/c9-5-3-1-2-4-6(5)8(13)11-10-7(4)12/h1-3H,9H2,(H,10,12)(H,11,13)
InChI key
HWYHZTIRURJOHG-UHFFFAOYSA-N
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General description
Luminol is a very efficient and commonly used chemiluminiscent compound in condensed phase. When basic solutions of luminol containing oxygen are treated with an oxidizing agent, light is produced. Oxidising agents capable of initiating this particular reaction are Nitrogen dioxide, PAN, hydrogen peroxide, ozone and other atmospheric oxidants.
Application
- As a chemiluminiscent substance. Used to measure opsonic and phagocytic function.
- As a biological sensor. Used to detect the polymorphonuclear leukocytes (PMNL) response in a patient with a myeloperoxidase (MPO) deficiency.
- Used as a forensic test for blood.
Luminol has been used in a protocol to study the immune-related responses in Arabidopsis thaliana via luminol/peroxidase-based assay.
Features and Benefits
- Very effective chemiluminiscent compound.
- Can detect sub-part per billion levels of metal ions with simple and inexpensive instrumentation.
Used for chemiluminescence analysis of, for example, metal cations, blood, and glucococorticoids.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Mechanism of cobalt catalysis of luminol chemiluminescence
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Analytical Chemistry, 47, 1639-1643 (1975)
T I Quickenden et al.
Luminescence : the journal of biological and chemical luminescence, 16(4), 295-298 (2001-08-21)
A wide range of domestic and industrial substances that might be mistaken for haemoglobin in the forensic luminol test for blood were examined. The substances studied were in the categories of vegetable or fruit pulps and juices; domestic and commercial
C S Easmon et al.
Immunology, 41(1), 67-74 (1980-09-01)
Luminol-dependent chemiluminescence produced by peripheral blood phagocytic cells was measured using a light detecting instrument, the Luminometer 1250 (LKB Wallac). Although less sensitive than liquid scintillation counters, the Luminometer can measure chemiluminescence effectively and detect experimentally induced and clinical variations
Fast gas chromatography with luminol chemiluminescence detection for the simultaneous determination of nitrogen dioxide and peroxyacetyl nitrate in the atmosphere
Marley, Nancy A., et al.
The Review of Scientific Instruments, 75, 4595-4605 (2004)
Luminol-based assay for detection of immunity elicitor-induced hydrogen peroxide production in Arabidopsis thaliana leaves.
Bisceglia NG, et al.
Bio-protocol, 5, e1685-e1685 (2015)
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