D199303

2,4-Dinitrophenylhydrazine

reagent grade, 97%

• Synonym(s): (2,4-dinitrophenyl)-hydrazine, DNPH
• Linear Formula: (O₂N)₂C₆H₃NHNH₂
• CAS Number: 119-26-6
• Molecular Weight: 198.14
• Beilstein: 615586
• MDL number: MFCD00007578
• UNSPSC Code: 12352100
• PubChem Substance ID: 24893586
• NACRES: NA.21

Properties

• grade: reagent grade
• Assay: 97%
• contains: 33% water (minimum)
• mp: 197-200 °C (lit.)
• SMILES string: NNc1ccc(cc1[N+]([O-])=O)[N+]([O-])=O
• InChI: 1S/C6H6N4O4/c7-8-5-2-1-4(9(11)12)3-6(5)10(13)14/h1-3,8H,7H2
• InChI key: HORQAOAYAYGIBM-UHFFFAOYSA-N

Description

General description

2,4-Dinitrophenylhydrazine (DNPH), a hydrazine derivative, is a potential mutagenic agent. Its molecular and crystal structure has been analyzed. The intra- and intermolecular hydrogen bonds in DNPH have been investigated by vibrational spectroscopic analysis. The mechanism of isothermal degradation of DNPH has been studied by differential scanning calorimetry (DSC).

Application

2,4-Dinitrophenylhydrazine has been used as a reagent to detect the presence of aldehydes and ketones in protein carbonyls. It may be used as a colorimetric reagent in the quantitative estimation of prednisone.

Packaging

500G contains total wet product weight of ~485G. Variance is due to addition of water to meet DOT requirements.

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H206,H302
• Precautionary Statements: P210 - P212 - P230 - P233 - P280 - P370 + P380 + P375 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Desen. Expl. 1
• Supplementary Hazards: EUH044
• Storage Class Code: 4.1A - Other explosive hazardous materials
• WGK: WGK 3
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves