840046P

Brain PI(4,5)P2

Avanti Research^™ - A Croda Brand

• Synonym(s): Brain PI(4,5)P2
• CAS Number: 383907-42-4
• UNSPSC Code: 12352211
• NACRES: NA.25

Properties

• description: L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine) (ammonium salt)
• Assay: >99% (TLC)
• form: powder
• packaging: pkg of 1 × 1 mg (840046P-1mg); pkg of 1 × 10 mg (840046P-10mg); pkg of 1 × 25 mg (840046P-25mg); pkg of 1 × 5 mg (840046P-5mg)
• manufacturer/tradename: Avanti Research^™ - A Croda Brand
• lipid type: phosphoglycerides
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: [H][C@@](COP(O[C@H]1[C@H](O)[C@@H](OP([O-])(O)=O)[C@H](OP(O)([O-])=O)[C@@H](O)[C@H]1O)([O-])=O)(OC(CCC/C=C\C/C=C\C/C=C\C/C=C\CCCCC)=O)COC(CCCCCCCCCCCCCCCCC)=O.[NH4+].[NH4+].[NH4+]
• InChI: 1S/C47H85O19P3.3H3N/c1-3-5-7-9-11-13-15-17-19-20-22-24-26-28-30-32-34-36-41(49)63-39(37-61-40(48)35-33-31-29-27-25-23-21-18-16-14-12-10-8-6-4-2)38-62-69(59,60)66-45-42(50)43(51)46(64-67(53,54)55)47(44(45)52)65-68(56,57)58;;;/h11,13,17,19,22,24,28,30,39,42-47,50-52H,3-10,12,14-16,18,20-21,23,25-27,29,31-38H2,1-2H3,(H,59,60)(H2,53,54,55)(H2,56,57,58);3*1H3/b13-11-,19-17-,24-22-,30-28-;;;/t39-,42?,43+,44?,45-,46?,47?;;;/m1.../s1
• InChI key: GAIOWTITBYPFJE-JVILRMJCSA-N

Description

General description

Although PI(4,5)P2 is a minor component of cell membranes, it plays a critical role as a substrate for a number of important signaling proteins. PI(4,5)P2 is an intermediate in the IP3/DAG pathway where it is hydrolyzed by phospholipase C to liberate the second messengers, inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). PI(4,5)P2 is also a substrate for PI 3-kinase where it is phosphorylated to PI(3,4,5)P3, an activator of downstream signaling components such as the protein kinase AKT.

Phosphatidylinositol 4,5-bisphosphate (PIP₂) is synthesized from the precursor phosphatidylinositol 4-phosphate(PI4P). PIP₂ is a phosphoinositide with three negative charges.

Application

Brain PI(4,5)P2 has been used:

• in the preparation of Folch liposome
• in the preparation of giant unilamellar vesicles and multilamellar lipid sheets
• as a substrate for rubidium flux assay
• in the biochemical reaction assay mixture for various lipid kinases like Phosphatidylinositol-3-kinase gamma (PI3K_γ), Phosphatidylinositol-3-kinase beta (PI3K_β), Phosphatidylinositol-3-kinase gamma (PI3K_α)

Biochem/physiol Actions

Phosphatidylinositol 4,5-bisphosphate (PIP₂) is a signaling phospholipid, present in the plasma membrane. It acts as a precursor for inositide 3 phosphate, diacylglycerol. PIP₂ is a gating ligand for most of the ion channels. It functions as an activatory ligand for eukaryotic inward rectifier potassium (Kir) channels.

Packaging

5 mL Clear Glass Sealed Ampule (840046P-10mg)

5 mL Clear Glass Sealed Ampule (840046P-1mg)

5 mL Clear Glass Sealed Ampule (840046P-25mg)

5 mL Clear Glass Sealed Ampule (840046P-5mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3