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05-858

Sigma-Aldrich

Anti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonal

clone 62-141-13, Upstate®, from rabbit

Synonym(s):

H4, Histone H4, H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

62-141-13, monoclonal

species reactivity

chicken, Saccharomyces cerevisiae, vertebrates, yeast, human

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable (ChIP-seq)
multiplexing: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

Saccharomyces cerevisiae ... Hhf1(852294)
human ... H4C1(8359)

General description

Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
This antibody recognizes histone H4 independant of any post-translational modifications.

Specificity

Broad species cross-reactivity expected based on sequence homology.
Recognizes Histone H4, Mr 10 kDa.

Immunogen

Epitope: a.a. 25-28
KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28.

Application

Anti-Histone H4 Antibody, pan, clone 62-141-13 is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex, ChIP-seq.
Epitope Mapping (using Luminex®):
1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).

Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Quality

Routinely evaluated by western blot.

Western Blot Analysis:
A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)

Target description

10 kDa

Physical form

Format: Purified
Protein A purified
Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

Luminex is a registered trademark of Luminex Corp
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The effects of repeated social defeat on long-term depressive-like behavior and short-term histone modifications in the hippocampus in male Sprague-Dawley rats.
Fiona Hollis,Hui Wang,David Dietz,Akash Gunjan,Mohamed Kabbaj
Psychopharmacology (Psychopharmacologia) null
Toggle involving cis-interfering noncoding RNAs controls variegated gene expression in yeast.
Bumgarner, SL; Dowell, RD; Grisafi, P; Gifford, DK; Fink, GR
Proceedings of the National Academy of Sciences of the USA null
Xue Zhou et al.
Cancer research, 70(10), 4214-4221 (2010-04-22)
Histone H3 lysine 4 (H3K4) trimethylation (H3K4me3) at the promoter region of genes has been linked to transcriptional activation. In the present study, we found that hypoxia (1% oxygen) increased H3K4me3 in both normal human bronchial epithelial Beas-2B cells and
Novel regulatory role for human Acf1 in transcriptional repression of vitamin D3 receptor-regulated genes.
Ewing, AK; Attner, M; Chakravarti, D
Molecular Endocrinology null
Lawryn H Kasper et al.
The EMBO journal, 29(21), 3660-3672 (2010-09-23)
It remains uncertain how the DNA sequence of mammalian genes influences the transcriptional response to extracellular signals. Here, we show that the number of CREB-binding sites (CREs) affects whether the related histone acetyltransferases (HATs) CREB-binding protein (CBP) and p300 are

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