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MilliporeSigma

1.11972

Millipore

GranuCult® Tryptose sulfite cycloserine agar (base)

ISO 7937, ISO 14189, APHA, suitable for microbiology

Synonym(s):

TSC agar

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About This Item

UNSPSC Code:
41106213
NACRES:
NA.74

Agency

APHA
ISO 14189
ISO 7937

Quality Level

sterility

non-sterile

form

granular

manufacturer/tradename

GranuCult® prime

technique(s)

microbiological culture: suitable

pH

7.4-7.8 (25 °C in H2O)

solubility

42 g/L

bulk density

660 kg/m3

application(s)

food and beverages
microbiology

General description

GranuCult® Tryptose sulfite cycloserine agar (base) is intended for the isolation and differentiation of Clostridium perfringens. The superior nutrient base provides optimal conditions for the development of Clostridia. Colonies producing hydrogen sulfide are characterized by blackening due to the reaction with sulfite and iron salt. Cycloserine inhibits the accompanying bacterial flora and may cause the colonies that develop to remain smaller. It also reduces diffusion and thus the disturbing blackening around the Clostridium perfringens colonies. TSC agar with MUP contains a fluorogenic substrate for the alkaline and acid phosphatase in addition to cycloserine 4-Methylumbelliferylphosphate (MUP). The acid phosphatase is a high specific indicator for Clostridium perfringens. It splits the fluorogenic substrate MUP, forming 4-methylumbelliferone, which can be identified by its fluorescence in long wave UV light. Thus, a strong suggestion for the presence of Clostridium perfringens can be obtained.

Application

GranuCult® Tryptose sulfite cycloserine agar (base) is intended for the isolation and differentiation of Clostridium perfringens from food and animal feed, water and other materials. TSC agar with MUP can be prepared using Clostridium perfringens Supplement (1.00888) to detect acid phosphatase by its fluorescence under UV light.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

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