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16-455

Sigma-Aldrich

Anti-Phosphoserine Antibody, clone 4A4, HRP Conjugate

clone 4A4, Upstate®, from mouse

Synonym(s):

4A4, anti-phosphoserine, phosphoserine antibody, pan-phosphoserine

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified antibody

antibody product type

primary antibodies

clone

4A4, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

phosphorylation (pSer)

General description

The identification of protein phosphorylation as a regulatory mechanism originated from studies by Fischer and Krebs in the mid 1950s that later earned them the 1992 Nobel prize. It is the major mechanism for the regulation of diverse cellular processes including cell division, protein synthesis, transcriptional regulation and neurotrans-mission. The steady state phosphorylation of any given substrate is governed by the opposing activities of kinases and phosphatases. It is now believed that a third of all eukaryotic cellular proteins are phosphorylated and that over 95% of all phosphorylation events occur on serine and threonine residues.

Specificity

Recognizes proteins containing phosphoserine residues, regardless of origin.

Immunogen

Epitope: phosphoserine
Phosphoserine conjugated to KLH.

Application

Anti-Phosphoserine Antibody, clone 4A4, HRP Conjugate is an antibody against Phosphoserine for use in WB.
Research Category
Signaling
Research Sub Category
General Post-translation Modification

Quality

Routinely evaluated by western blot on RIPA lysates from insulin-treated human HEK293 cells.

Target description

Varies

Physical form

100 μL of protein G purified mouse IgG1 in PBS containing 0.05% Kathon® and HRP stabilizing compounds. Sufficient for a minimum of 10 immunoblots, if used at the optimum concentration of 1:4000 in 10 mL blocking buffer. Liquid.
Protein G Purified

Storage and Stability

6 months at 2-8°C from date of shipment.
NOTE: DO NOT FREEZE. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

Kathon is a registered trademark of Rohm and Haas Co.
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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