344206-M

Formamide, Molecular Biology Grade

Deionized. Adenine deaminase inhibitor. Used in DNA stripping during genetic fingerprinting techniques.

• Synonym(s): Formamide, CH₃NO, Amide C₁, Formic amide
• Linear Formula: HCONH₂
• CAS Number: 75-12-7
• Molecular Weight: 45.04
• Beilstein: 505995
• MDL number: MFCD00007941
• UNSPSC Code: 12352111
• NACRES: NA.28

Properties

• grade: Molecular Biology
• Quality Level: 100
• vapor density: 1.55 (vs air)
• vapor pressure: 0.08 mmHg ( 20 °C); 30 mmHg ( 129 °C)
• form: liquid
• autoignition temp.: 932 °F
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• technique(s): hybridization: suitable
• refractive index: n20/D 1.447 (lit.)
• pH: 8-10 (20 °C, 200 g/L)
• bp: 210 °C (lit.)
• mp: 2-3 °C (lit.)
• solubility: water: 20 mg/mL (~0.5 M)
• density: 1.134 g/mL at 25 °C (lit.)
• cation traces: heavy metals (as Pb): ≤0.0001%
• foreign activity: DNases, proteases, RNases, none detected
• storage temp.: 10-30°C
• SMILES string: NC=O
• InChI: 1S/CH3NO/c2-1-3/h1H,(H2,2,3)
• InChI key: ZHNUHDYFZUAESO-UHFFFAOYSA-N

Description

General description

Deionized. Adenine deaminase inhibitor. Used in DNA stripping during genetic fingerprinting techniques.

Useful as an ionizing solvent. Absorbance (0.5 M): ≤0.08 at 260 nm; ≤0.03 at 280 nm. Adenine deaminase inhibitor. Used in DNA stripping during genetic fingerprinting techniques. Supplied as deionized.

Application

Formamide destabilizes nucleic acid duplexes and may be used, typically, at a concentration of 50%, in hybridization protocols requiring lower hybridization temperatures.

Warning

Toxicity: Carcinogenic / Teratogenic (D)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H351,H360D,H373
• Precautionary Statements: P202 - P260 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Carc. 2 - Repr. 1B - STOT RE 2 Oral
• Target Organs: Blood
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point(F): 305.6 °F
• Flash Point(C): 152 °C