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444805

Sigma-Aldrich

Membrane Traffic Inhibitor, A5

The Membrane Traffic Inhibitor, A5 controls the biological activity of adaptor complex AP-1.

Synonym(s):

Membrane Traffic Inhibitor, A5, 1-(4-(4-(E-2-Methyl-2-butenyl)piperazinyl)phenyl)ethanone, HCl, 5431942, A5

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About This Item

Empirical Formula (Hill Notation):
C17H24N2O · xHCl
Molecular Weight:
272.39 (free base basis)
UNSPSC Code:
12352200
NACRES:
NA.77

Quality Level

Assay

≥97% (HPLC)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)
protect from light

color

pale yellow

solubility

water: 1 mg/mL

shipped in

ambient

storage temp.

−20°C

General description

A cell-permeable piperazinyl compound that specifically blocks clathrin adaptor complex AP-1-dependent traffic between trans-Golgi network (TGN) and endosomes in budding yeast, while exhibiting little effects against other trafficking pathways involving TGN and endosomes. Inhibits growth of yeast cells lacking complementary GGA1/2 adaptor proteins (EC50 = 50 µM), but not wild-type or AP-1-deficient cells even at concentrations as high as 300 µM. A5 presumably acts at a stage after AP-1 membrane recruitment as evidenced by an enhanced AP-1 perinuclear localization upon A5 treatment of HeLa cells.

Packaging

Packaged under inert gas

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Duncan, M.C., et al. 2007. Proc. Natl. Acad. Sci. USA104, 6235.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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