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612161

Sigma-Aldrich

Anti-Tissue Factor Mouse mAb (TF9-10H10)

lyophilized, clone TF9-10H10, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

TF9-10H10, monoclonal

form

lyophilized

does not contain

preservative

species reactivity

primate, human

should not react with

rabbit

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

isotype

IgG1

shipped in

ambient

storage temp.

−20°C

target post-translational modification

unmodified

General description

Purified mouse monoclonal antibody (see application references). Recognizes the tissue factor protein.
Recognizes the tissue factor protein.
This Anti-Tissue Factor Mouse mAb (TF9-10H10) is validated for use in Dot Blot, ELISA, FC, Frozen Sections, WB, IF, Paraffin Sections, Neutralization Studies for the detection of Tissue Factor.

Immunogen

Epitope: within extracellular domain
Human
purified apoprotein of human tissue factor

Application

Dot Blot (see application references)

ELISA (see application references)

Flow Cytometry (see application references)

Frozen Sections (see application references)

Immunoblotting (1:1000, see application references)

Immunofluorescence (see application references)

Paraffin Sections (see application references)

Neutralization Studies (not recommended)

Warning

Toxicity: Standard Handling (A)

Physical form

Lyophilized from 100 mM mannitol, 150 mM PBS, pH 7.4.

Reconstitution

Reconstitute lyophilized antibody with 0.5 ml sterile dH₂O. Aliquot and freeze (-20°C) for long-term storage. For short-term storage at 4°C, add 0.02% sodium azide. Avoid freeze/thaw cycles of solutions.

Other Notes

Specific for epitope locus I. Recognizes reduced and native, non-reduced human and primate tissue factor. This antibody does not inhibit coagulation or neutralize factor VII binding to TF. Significant reactivity with tissue factor from species other than human and primate has not been established. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Human reproduction (Oxford, England), 27(5), 1300-1313 (2012-03-10)
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Cell chemical biology, 24(7), 858-869 (2017-07-04)
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