Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

AP202P

Sigma-Aldrich

Goat Anti-Rat light chain Antibody, HRP conjugate

Chemicon®, from goat

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

goat

Quality Level

conjugate

peroxidase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

unmodified

General description

Light chains covalently bound to a pair of heavy chains to form an antibody molecule. Immunoglobulin light chains occur in two types, kappa or lambda. Light chain′s mass is about 25,000 Daltons per mole. Each light chain consists of about 250 amino-acids long. These are usually designated by the Greek letters kappa and lambda. The ratio of kappa to lambda found in the immunoglobulin population varies by species.

Specificity

Minimal cross-reaction with bovine, goat, horse, mouse, human, rabbit, and sheep.
The antibody reacts strongly with native primary antibodies primarily with kappa light chains. It is not suitable for detecting lambda light chains. The antibody does not react with the heavy chain of rat IgG. The antibody has been tested by ELISA and adsorbed to ensure minimal cross-reaction with bovine, goat, horse, mouse, human, rabbit, and sheep immunoglobulins.

Immunogen

Epitope: Kappa light chain
Prepared from purified rat IgG light chain.

Application

Goat anti-Rat light chain Antibody, HRP conjugate is an antibody against light chain for use in ELISA, WB, IC, IH.
Research Category
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Western Blotting: 1:5,000 -1:100,000 with chromogenic substrates
1:10,000 -1:200,000 with ECL substrates
ELISA: 1:5,000 -1:100,000
Immunohistochemistry: 1:500 - 1:5,000
Immunocytochemistry: 1:500 - 1:5,000
Optimal working dilutions must be determined by the end user.

Target description

25 kDa

Physical form

ImmunoAffinity Purified
Purified by immunoaffinity chromatography. Lyophilized in 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6. 15 mg/mL BSA as stabilizer.

Storage and Stability

Maintain lyophilized product at 2°-8°C for up to 12 months. Reconstitute vial with of distilled water (addition of 0.5 ml water will yield a final concentration of 1 mg/mL). After reconstitution the product is stable for six weeks at 2°-8°C in the dark as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze thaw cycles. Keep away from light.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Characterisation and prognostic value of tertiary lymphoid structures in oral squamous cell carcinoma.
Wirsing, AM; Rikardsen, OG; Steigen, SE; Uhlin-Hansen, L; Hadler-Olsen, E
BMC Clinical Pathology null
Anna M Wirsing et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 37(2), 2449-2459 (2015-09-19)
Staging of oral squamous cell carcinoma is based on the tumour-node-metastasis (TNM) system, which has been deemed insufficient for prognostic purposes. Hence, better prognostic tools are needed to reflect the biological diversity of these cancers. Previously, high numbers of specialized
Shutang Tan et al.
Nature plants, 6(5), 556-569 (2020-05-13)
Directional intercellular transport of the phytohormone auxin mediated by PIN-FORMED (PIN) efflux carriers has essential roles in both coordinating patterning processes and integrating multiple external cues by rapidly redirecting auxin fluxes. PIN activity is therefore regulated by multiple internal and
Anna Maria Wirsing et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 31(6), 910-922 (2018-02-09)
Oral squamous cell carcinomas are associated with a poor prognosis, which may be partly due to functional impairment of the immune response. Lymphocyte recruitment to the tumor site is facilitated by high-endothelial venules, whereas expression of programmed-death ligand 1 (PD-L1)
Christophe Chapard et al.
Molecular cell, 75(2), 224-237 (2019-06-16)
Cohesin entraps sister DNAs within tripartite rings created by pairwise interactions between Smc1, Smc3, and Scc1. Because Smc1/3 ATPase heads can also interact with each other, cohesin rings have the potential to form a variety of sub-compartments. Using in vivo cysteine

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service