EZCL-31K

Canine Leptin ELISA Kit

measures Leptin in 20 μL serum, plasma or cell culture supernatent

• Synonym(s): Canine Leptin Kit, Leptin ELISA
• UNSPSC Code: 12161503
• NACRES: NA.84

Properties

• product name: Canine Leptin ELISA,
• species reactivity: canine
• Quality Level: 200
• packaging: kit of 1 × 96 wells
• parameter: 20 μL sample volume (4hr assay)
• technique(s): ELISA: suitable
• input: sample type serum; sample type plasma (K2 EDTA)
• application(s): research use
• assay range: inter-assay cv: 6-7%; intra-assay cv: 2-6%; sensitivity: 0.21 ng/mL; standard curve range: 0.78-50 ng/mL; accuracy: 100-106%
• detection method: colorimetric (450nm/590nm)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Leptin, a 16 kD protein and a product of the obesity (ob) gene, is synthesized and secreted predominantly by adipose tissue and is, therefore, considered an adipokine. With circulating levels directly proportional to the total amount of body fat, leptin plays an important role in whole body metabolism, regulating food intake and energy balance, as well as assisting in the control of homeostasis, reproductive function, the immune system and numerous other body functions. Binding to receptors of the hypothalamus, leptin signals food satiety and reduces appetite, although evidence suggests that, in general, obese people may have unusually high circulating levels of leptin. This may indicate a resistance to leptin similar to the insensitivity to insulin associated with type 2 diabetes. Consequently, the study of leptin is essential to understanding the relationship among appetite, energy balance and obesity.

Specificity

Used to detect/quantify: Leptin

Application

This Canine Leptin ELISA is used to measure & quantify Leptin levels in Metabolism & Endocrine & Neuroscience research.

This Is a 4-hour room temperature assay using 20 μL sample size

Research Category: Metabolism

Research Sub-Category: Obesity, Metabolic Disorders

Packaging

96-well strip plate

Other Notes

Contact Technical Support.

Disclaimer

For research use only. Not for use in diagnostic procedures.