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FCMAB133F

Sigma-Aldrich

Milli-Mark® Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate

clone TRA-2-49/6E, Milli-Mark®, from mouse

Synonym(s):

Anti-TRA-2-49 FITC, Clone TRA-2-49 Antibody, TRA-2-49 Antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

FITC conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

TRA-2-49/6E, monoclonal

species reactivity

human

manufacturer/tradename

Milli-Mark®

technique(s)

flow cytometry: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

General description

In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Specificity

Antibody recognizes TRA-2-49.

Immunogen

2102Ep human Embryonal Carcinoma Cells
Epitope: Unknown

Application

Working Solution Cells / test Total Reaction Volume
5 μl 5 x 105 Cells / 95 μl PBS 100 μl
10 μl 1 x 106 Cells / 90 μl PBS 100 μl
Detect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Quality

Evaluated by flow cytometry using 2102 Ep cells.

Target description

212/80/55 kDa Observed

Physical form

Protein A purified
Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
2102 Ep cells.

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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