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MABE1151

Sigma-Aldrich

Anti-OASIS (CREB3L1) Antibody, clone 10H1

clone 10H1, from mouse

Synonym(s):

Cyclic AMP-responsive element-binding protein 3-like protein 1, cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, OASIS

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10H1, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology), rat (based on 100% sequence homology), bovine (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... CREB3L1(90993)

General description

Cyclic AMP-responsive element-binding protein 3-like protein 1 (UniProt: Q96BA8; also known as cAMP-responsive element-binding protein 3-like protein 1, Old astrocyte specifically-induced substance, OASIS) is encoded by the CREB3L1 (also known as OASIS, PSEC0238) gene (Gene ID: 90993) in human. OASIS is a single-pass type II membrane protein that serves as a transcription factor and is involved in
unfolded protein response (UPR). It binds the DNA consensus sequence 5′-GTGXGCXGC-3′. It has a cytoplasmic domain (aa 1-374), a helical domain (aa 375-395), and a luminal domain (aa 396-519). In the absence of endoplasmic reticulum (ER) stress, it is inserted into ER membranes, with N-terminal DNA-binding and transcription activation domains oriented toward the cytosolic face of the membrane. However, in response to ER stress, it is transported to the Golgi, where it is cleaved in a site-specific manner by resident proteases S1P/MBTPS1 and S2P/MBTPS2. The released N-terminal cytosolic domain is translocated to the nucleus to effect transcription of specific target genes. OASIS also plays a critical role in bone formation through the transcription of COL1A1, and possibly COL1A2, and the secretion of bone matrix proteins. It directly binds to the UPR element (UPRE)-like sequence in an osteoblast-specific COL1A1 promoter region and induces its transcription. OASIS is also required to protect astrocytes from ER stress-induced cell death. In astrocytes, it binds to the cAMP response element (CRE) of the BiP/HSPA5 promoter and participate in its transcriptional activation. Mutations in CREB3L1 gene are reported to cause osteogenesis imperfecta, a connective tissue disorder characterized by low bone mass, bone fragility and susceptibility to fractures after minimal trauma.

Specificity

Clone 10H1 detects Cyclic AMP-responsive element-binding protein 3-like protein 1 (CREB3L1) in human tissues. it targets an epitope within 35 amino acids from the N-terminal region.

Immunogen

KLH-conjugated linear Peptide corresponding to 35 amino acids from the N-terminal region of human OASIS (CREB3L1).

Application

Anti-OASIS (CREB3L1), clone 10H1, Cat. No. MABE1151, is a highly specific mouse monoclonal antibody that targets Cyclic AMP-responsive element-binding protein 3-like protein 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected OASIS (CREB3L1) in human pancreas and human tonsil tissues.

Immunohistochemistry Analysis: A representative lot detected OASIS (CREB3L1) in Immunohistochemistry applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233).

Western Blotting Analysis: A representative lot detected OASIS (CREB3L1) in Western Blotting applications (Denard, B., et. al. (2015). PLoS One. 10(6):e0129233; Chen, Q., et. al. (2016). Mol Cell. 63(4):567-78).

Quality

Evaluated by Immunohistochemistry in human prostate cancer tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected OASIS (CREB3L1) in human prostate cancer tissue sections.

Target description

57.00 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Daniele Pittari et al.
Frontiers in cell and developmental biology, 10, 986997-986997 (2022-11-01)
Upon progesterone stimulation, Endometrial Stromal Cells (EnSCs) undergo a differentiation program into secretory cells (decidualization) to release in abundance factors crucial for embryo implantation. We previously demonstrated that decidualization requires massive reshaping of the secretory pathway and, in particular, of

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