MABF2767

Anti-MERS-CoV NP Antibody

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: #20, monoclonal
• mol wt: calculated mol wt 25.05 kDa; observed mol wt ~45 kDa
• species reactivity: virus
• packaging: antibody small pack of 100 μg
• technique(s): ELISA: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG2b
• UniProt accession no.: R9UM87
• shipped in: dry ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: Unknown ... N nucleocapsid protein(14254601)

Description

General description

Nucleoprotein (UniProt: R9UM87; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 14254601) in Middle East respiratory syndrome-related coronavirus (MERS-CoV). The MERS-CoV is a positive-strand RNA virus of the betacoronavirus genus within the Coronaviridae family that causes severe respiratory syndrome in human. The mature MERS-CoV contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). Nucleoprotein of MERS-CoV is a homooligomeric multifunctional protein that plays roles in genome encapsidation, RNA synthesis during replication, host translational shutoff, and innate immunity disruption. It packages the positive strand viral genome RNA into helical ribonucleocapsid and is involved in virion assembly through its interactions with the viral genome and membrane protein M. The nucleoprotein is reportedly organized into two folded domains known as the N-terminal domain (NTD) and the C-terminal domain (CTD) that are separated by a disordered region (LKR) containing a serine/ arginine stretch. Clone 46 displays specificity for MERS-CoV nucleoprotein and does not show cross-reactivity with nucleoprotein from other related viruses, including SARS coronavirus. (Ref.: Nguyen, THV., et al. (2019). Acta Crystallogr. D. Struct. Biol. 75(1); 8-15; Yamaoka, Y., et al. (2016). Front. Microbiol. 7; Article 509).

Specificity

Clone 20 is a mouse monoclonal antibody that detects nucleoprotein of Middle East respiratory syndrome-related coronavirus (MERS-CoV). It target an epitope within 6 amino acids from the C-terminal region.

Immunogen

His-tagged recombinant fragment corresponding to 292 amino acids (122-413) from the nucleoprotein of Middle East respiratory syndrome-related coronavirus (MERS-CoV).

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant MERS-CoV nucleoprotein

Western Blotting Analysis: A 1:1,000,000 dilution of this antibody detected recombinant Middle East respiratory syndrome-related coronavirus (MERS-CoV) Nucleoprotein.

Tested Applications

Western Blotting Analysis: A representative lot detected MERS-CoV NP in Western Blotting applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Immunoprecipitation Analysis: A representative lot immunoprecipitated MERS-CoV NP in Immunoprecipitation applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

ELISA Analysis: A representative lot detected MERS-CoV NP in ELISA applications (Yamaoka, Y., et. al. (2016). Front Microbiol. 7:509).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-MERS-CoV NP, clone 20, Cat. No. MABF2767, is a mouse monoclonal antibody that detects nucleoprotein in Middle East respiratory syndrome-related coronavirus (MERS-CoV) and is tested for use in ELISA, Immunoprecipitation, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C . Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable