MABF949-SF

Anti-Rabies Virus Antibody, Serum Free Antibody, clone 1112-1

clone 1112-1, from mouse

• Synonym(s): Glycoprotein, Glycoprotein Rabies virus
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1112-1, monoclonal
• species reactivity: rabies virus, virus
• packaging: antibody small pack of 25 μg
• technique(s): ELISA: suitable; flow cytometry: suitable; neutralization: suitable
• isotype: IgG1κ
• UniProt accession no.: P03524
• target post-translational modification: unmodified

Description

General description

Glycoprotein (UniProt: P03524) is encoded by the G gene in Rabies virus, strain ERA. Glycoprotein is a single-pass type I membrane protein that belongs to the lyssavirus glycoprotein family. It attached the virus to host cellular receptor, inducing endocytosis of the virion. In the endosome, the acidic pH induces conformational changes in the glycoprotein trimer, which trigger fusion between virus and cell membrane. The muscular form of the nicotinic acetylcholine receptor (nAChR), the neuronal cell adhesion molecule (NCAM), and the p75 neurotrophin receptor (p75NTR) are reported to bind glycoprotein and thereby facilitate rabies virus entry into cells. Glycoprotein is synthesized with a signal peptide (aa 1-19), which is subsequently cleaved off in the mature form. Its structure includes a virion surface (aa 20-459), helical region (aa 460-480) and an intravirion (aa 481-524). Arginine 352 is reported to be involved in Rabies virus pathogenicity and its mutation attenuates the virus. Glycoprotein is glycosylated and palmitoylated by host and glycosylation is considered to be crucial for glycoprotein export at the cell surface. Clone 1112-1 is a serum-free version and is shown to neutralize Rabies Virus, CVS-11 strain. (Ref.: Dietzschold, B et al. (1992). Proc. Natl. Acad. Sci. USA 89 (15); 7252-7256; Wiktor, TJ and Koprowski, H (1980). J. Exp. Med. 152: 99-112; Marissen, WE et al (2005). J. Virol. 79(8): 4672-4678).

Specificity

Clone 1112-1 is a mouse monoclonal antibody that detects detects glycoprotein of strain ERA rabbies virus.

Immunogen

Protein G of Evelyn Rokitniki Abelseth(ERA) SAD derived RABV strain).

Application

Anti-Rabies Virus, Serum Free, clone 1112-1, Cat. No. MABF949-SF, is a mouse monoclonal antibody that detects Glycoprotein of rabies virus., strain ERA and has been tested for use in ELISA, Flow Cytometry, and Neutralizing applications.

Neutralizing Analysis: A representative lot neutralized Rabies Virus, Serum Free CVS-11 strain in Neutralizing applications (Bunschoten, H., et. al. (1989). J Gen. Virol. 70 ( Pt 2):291-8; Dietzschold, B., et. al. (1992). Proc Natl Acad Sci USA. 89(15):7252-6; Muller, T., et. al. (2009). PLoS Negl Trop Dis. 3(11):e542; Schumacher, C.L., et. al. (1989). J Clin Invest. 84(3):971-5).

ELISA Analysis: A representative lot detected Rabies Virus, Serum Free in ELISA applications (Morgeaux, S., et. al. (2017). Vaccine. 35(6):966-971).

Quality

Evaluated by Flow Cytometry in L929 cells expressing Rabies virus glycoprotein.

Flow Cytometry Analysis: 2 µg of this antibody detected Rabies Virus, Serum Free in L929 cells expressing Rabies virus glycoprotein.

Target description

58.66 kDa calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.