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MABT110

Sigma-Aldrich

Anti-Aggrecan (neoepitope) Antibody, clone BC-3

clone BC-3, from mouse

Synonym(s):

Aggrecan core protein, Cartilage-specific proteoglycan core protein, CSPCP, Chondroitin sulfate proteoglycan core protein 1, Chondroitin sulfate proteoglycan 1, Aggrecan core protein 2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BC-3, monoclonal

species reactivity

bovine, human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ACAN(176)

General description

Aggrecan (CSPG1, CSPCP, or CSPGCP) is a chondroitin-sulfate proteoglycan belonging to the family of letican proteins. Aggrecan is a large globar structure containing multiple domains including carbohydrate-recognition domains; complement binding protein domains; immunoglobulin domains; proteoglycan tandem repeats; and in some cases, EGF-like domains. It can be degraded by aggrecanase. The N-terminus of aggrecan binds ECM hyaluronan in the presence of the linking protein, and forms complex aggregates, whereas the C-terminus binds a number of other ECM proteins including tenascins, glycolipids, and fibulins. Aggrecan is a major component of cartilage tissue and it enables this tissue to withstand compression by maintaining the viso-elastic properties of cartilage. Aggrecan may also facilitate neural maturation and plasticity by contributing to the formation of perineuronal nets in the developing CNS.

Specificity

This antibody detectes the Args neoepitope sequence in aggrecanase-cleaved aggrecan (Tortella, M. D., et al. (1999). Science. 284(5420):1664-1666.).

Immunogen

Epitope: Args neoepitope sequence
Linear peptide corresponding to human Aggrecan neoepitope.

Application

Anti-Aggrecan (neoepitope) Antibody, clone BC-3 detects level of Aggrecan (neoepitope) & has been published & validated for use in Western Blotting & IHC.
Research Category
Cell Structure
Research Sub Category
ECM Proteins
Western Blot Analysis: A representative lot detected Aggrecan neoepitope in human chondrocite cell lysate.

Quality

Evaluated by Western Blot in SW1353 cell lysate.

Western Blot Analysis:1 µg/mL of this antibody detected Aggrecan neoepitope in 10 µg of SW1353 cell lysate.

Target description

~60 kDa observed. Uniprot describes three isoforms at ~250 kDa, ~246 kDa and ~239 kDa Aggrecan is degraded by aggrecanase into a ~60 kDa protein and other higher molecular weight products (Lemons, M. L. et al. (2001). J Neurosci. 21(13):4772-4781. Forsang, A. J. et al. (1995). Biochem J. 310(Pt 1):337-343.).

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
SW1353 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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