MABT25

Anti-Myosin 2 Antibody, clone 5B11.1

clone 5B11.1, from mouse

• Synonym(s): Myosin-2, Myosin heavy chain 2, Myosin heavy chain 2a, MyHC-2a, Myosin heavy chain Iia, MyHC-Iia, Myosin heavy chain, skeletal muscle, adult 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 5B11.1, monoclonal
• species reactivity: mouse, human, rat
• technique(s): immunohistochemistry: suitable; western blot: suitable
• isotype: IgG3κ
• NCBI accession no.: NP_060004
• UniProt accession no.: Q9UKX2
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... MYH2(4620)

Description

General description

Myosin is a hexameric protein consisting of two heavy chains including Myosin-2 (MYH2), two regulatory light chains, and two alkali light chains. The myosin-2 heavy chain interacts with the actin cytoskeleton and mediates muscle contractility in eukaryotic cells. Defects in myosin-2 result in inclusion body myopathy type 3.

Immunogen

KLH-conjugated linear peptide corresponding to mouse Myosin 2.

Application

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Myosin 2 Antibody, clone 5B11.1 is validated for use in Western Blotting, IHC for the detection of Myosin 2.

Western Blot Analysis: 0.5 µg/mL from a representative lot detected Myosin-2 in L6 cell lysate (Myosin may be observed as a smear in some cell lysates).

Western Blot Analysis: A representative lot detected Myosin-2 in mouse heart cell cytosolic extracts.

Quality

Evaluated by Immunohistochemistry in human cardiac muscle tissue.

Immunohistochemistry Analysis: A 1:300 dilution of this antibody detected Myosin-2 in human cardiac muscle tissue.

Target description

~220 kDa observed

Physical form

Format: Purified

Purified mouse monoclonal IgG3κ in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human cardiac muscle tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable