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11449460001

Roche

DNA Molecular Weight Marker IX

solution, pkg of 50 μg (in 200 μl)

Synonym(s):

DNA marker

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About This Item

UNSPSC Code:
41105335

form

solution

Quality Level

packaging

pkg of 50 μg (in 200 μl)

manufacturer/tradename

Roche

concentration

250 μg/mL

color

colorless

solubility

water: miscible

storage temp.

−20°C

Related Categories

General description

DNA Molecular Weight Marker IX contains a mixture of eleven DNA fragments. The mixture is obtained by cleavage of φX 174 DNA with restriction endonuclease Hae III.
Size Range: 72 to 1353 bp

Application

DNA Molecular Weight Marker IX is especially designed for the analysis of mid-size PCR products in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:
  • Restriction digests
  • PCR
  • RT-PCR

Sequence

The mixture contains eleven DNA fragments with the following base pair lengths (1 base pair = 660 Daltons): 72, 118, 194, 234, 271, 281, 310, 603, 872, 1078, and 1353.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Unit Definition

50 μg = 1A260 unit

Preparation Note

Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

does not flash

Flash Point(C)

does not flash


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C N Okeke et al.
Journal of clinical microbiology, 39(10), 3491-3494 (2001-09-28)
The LightCycler system (two-step reverse transcription-PCR-fluorescent hybridization [LC RT-PCR-FH]) was used to quantify Candida albicans actin mRNA as a means of assessing its viability in a reconstituted skin model of cutaneous candidiasis following the application of an antimycotic. A 192-bp
Patrizia Spigaglia et al.
Journal of medical microbiology, 53(Pt 11), 1129-1136 (2004-10-22)
Recent studies have shown that Clostridium difficile strains with variant toxins and those with resistance to macrolide-lincosamide-streptogramin B (MLSB) are increasingly causing severe disease and outbreaks in hospital settings. Here, the pathogenicity locus (PaLoc), the acquisition of binary toxin, and

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