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P0290

Sigma-Aldrich

Phenol red solution

0.5%, liquid, sterile-filtered, BioReagent, suitable for cell culture

Synonym(s):

Phenolsulfonphthalein solution

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About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352207
PubChem Substance ID:
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

product line

BioReagent

form

liquid

concentration

0.5%

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

SMILES string

O=S1(C2=CC=CC=C2C(C3=CC=C(O)C=C3)(C4=CC=C(O)C=C4)O1)=O

InChI

1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H

InChI key

BELBBZDIHDAJOR-UHFFFAOYSA-N

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General description

Phenol red belongs to the class of triphenylmethane dyes and is widely used in the textile industry.

Application

Phenol red solution has been used:
  • as a tracer dye for microinjection of zebrafish eggs
  • as a component of the mMW medium for the preparation of mouse embryos
  • in the preparation of versene-ethylenediamine tetraacetic acid (EDTA) solution
  • as a pH indicator in Dulbecco′s modified Eagle medium (DMEM) during glycolytic stress (GST) tests and mitochondrial stress tests (MST) to evaluate metabolic function in cells.

Biochem/physiol Actions

Phenol red solution acts as a pH indicator for the culture medium.Phenol red solution acts as a pH indicator for the culture medium that turns yellow at a pH of 6.4 or lower and changes into red at a pH of 8.2 or higher. It enables a fast and simple assessment of the culture′s health. In addition to its application as a pH indicator in culture mediums, Phenol red is used to detect human peroxidase enzyme activity, as well as cellular hydrogen peroxide levels, in various experiments.

Features and Benefits

High-quality, endotoxin-tested solution, suitable for mammalian cell culture.

Preparation Note

Prepared in Dulbecco′s Phosphate Buffered Saline.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Natacha Merindol et al.
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 128, 104423-104423 (2020-05-18)
Rapid and reliable screening of SARS-CoV-2 is fundamental to assess viral spread and limit the pandemic we are facing. In this study, we compared direct rRT-PCR method (without RNA extraction) using SeeGene AllplexTM 2019-nCoV rRT-PCR with the RealStar® SARS-CoV-2 rRT-PCR
Shivshankar R Mane et al.
Small (Weinheim an der Bergstrasse, Germany), 14(36), e1801571-e1801571 (2018-08-07)
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Mutations that accumulate in self-renewing hematopoietic stem and progenitor cells (HSPCs) can cause severe blood disorders. To model such disorders in mice, we developed a CRISPR/Cas9/adeno-associated virus (AAV)-based system to knock in and repair genes by homologous recombination in mouse
S F Gabby Krens et al.
BMC genomics, 9, 196-196 (2008-04-30)
The MAPK signaling proteins are involved in many eukaryotic cellular processes and signaling networks. However, specific functions of most of these proteins in vertebrate development remain elusive because of potential redundancies. For instance, the upstream activation pathways for ERK1 and
P K J D de Jonge et al.
Cancer immunology, immunotherapy : CII, 72(10), 3323-3335 (2023-07-21)
Allogeneic natural killer (NK) cell-based immunotherapy is a promising, well-tolerated adjuvant therapeutic approach for acute myeloid leukemia (AML). For reproducible NK cell immunotherapy, a homogenous, pure and scalable NK cell product is preferred. Therefore, we developed a good manufacturing practice

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