11012009

ESO26

• Synonym(s): ESO-26 Cells
• UNSPSC Code: 41106514

Properties

• biological source: human esophagus
• growth mode: Suspension
• karyotype: Not specified
• morphology: Large spherical clumps of cells loosely adherent to the plastic
• products: P53 mutation c.742C>T
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Adenocarcinoma of the gastroesophageal junction

Cell Line Description

ESO26 was established from a primary tumour located in the gastroesophageal junction and distal oesophagus of a 56 year-old Caucasian male in 2000 involving lymph nodes and distant metastases. ESO26 was found to be tumourigenic in female NMRI nude mice or NOD-SCID mice.

DNA Profile

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 9,10
D13S317: 9,11
D16S539: 9
D5S818: 12
D7S820: 11
THO1: 9
TPOX: 7,9
vWA: 14,18

Culture Medium

RPMI-1640 + 2 mM Glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 2-9 x 10⁵ cells/cm³. Cells are loosely adherent and can be dislodged by gentle tapping or use of 0.02% EDTA solution. 5% CO₂; 37 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable