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61683

Sigma-Aldrich

Atto 540 Q NHS ester

BioReagent

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About This Item

MDL number:
MFCD07370567
UNSPSC Code:
12352200
NACRES:
NA.32
Pricing and availability is not currently available.

product line

BioReagent

Assay

≥90% (coupling to amines)

manufacturer/tradename

ATTO-TEC GmbH

technique(s)

UV/Vis spectroscopy: suitable

suitability

corresponds for UV test

storage temp.

−20°C

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5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate BioReagent, suitable for fluorescence, ≥85% (HPCE)

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5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate

suitability

suitable for fluorescence

suitability

-

suitability

-

suitability

suitable for fluorescence

fluorescence

λex 550 nm; λem 567 nm in phosphate buffer/SDS pH 7.0

fluorescence

-

fluorescence

λex 559 nm; λem 575 nm in methanol

fluorescence

λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase)

solubility

DMF: soluble, methanol: soluble, DMSO: soluble

solubility

-

solubility

chloroform/methanol: 10 mg/mL, clear

solubility

DMF: soluble, DMSO: soluble, methanol: soluble

Quality Level

100

Quality Level

100

Quality Level

-

Quality Level

100

assay

≥98.0% (TLC)

assay

97%

assay

98%

assay

≥85% (HPCE)

product line

BioReagent

product line

-

product line

-

product line

BioReagent

Application

Atto 540Q is a new quencher for fluorescence characterized by high molecular absorption (105.000). Atto 540Q is characterized by a high photostability and thermostability. It is moderately hydrophilic, and soluble in polar solvents as DMF or DMSO. Atto 540 Q NHS ester is used to label molecules such as proteins via conjugation to primary amine groups.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCB5049
1463590

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Switching modulation for protein labeling with activatable fluorescent probes.
Kalyan K Sadhu et al.
Chembiochem : a European journal of chemical biology, 12(9), 1299-1308 (2011-06-02)
Daniela C Dieterich
Current opinion in neurobiology, 20(5), 623-630 (2010-07-24)
Fluorescent proteins have revolutionized cell biology and, therefore, our understanding of the complex molecular and cellular mechanisms that wire the brain together and enable its plasticity throughout life. The ability to visualize cell biological processes has inspired the development of
Gražvydas Lukinavičius et al.
Current opinion in chemical biology, 15(6), 768-774 (2011-11-15)
Numerous synthetic fluorophores have been developed that can switch their spectroscopic properties upon interaction with other molecules or by irradiation with light. In recent years, protein-labeling techniques have been introduced that permit the specific attachment of such molecules to proteins

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