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P1379

Sigma-Aldrich

TWEEN® 20

viscous liquid

Synonym(s):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
CAS Number:
MDL number:
NACRES:
NA.21

description

non-ionic

Quality Level

form

viscous liquid

mol wt

~1228

composition

lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)

concentration

≥40.0% (GC)

technique(s)

DNA extraction: suitable
RNA extraction: suitable
cell culture | mammalian: suitable
protein purification: suitable

impurities

≤3.0% water

refractive index

n20/D 1.468 (lit.)

CMC

0.06 mM (20-25°C)

acid number

≤2.2 mg/g

hydroxyl value

96‑108 mg/g

transition temp

cloud point 76 °C

density

1.095 g/mL at 25 °C (lit.)

HLB

16.7

suitability

complies for IR spectroscopy

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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TWEEN® 20

description

non-ionic

description

non-ionic

description

non-ionic

description

non-ionic

form

viscous liquid

form

viscous liquid

form

viscous liquid

form

viscous liquid

mol wt

~1228

mol wt

~1228

mol wt

~1228

mol wt

~1228

technique(s)

DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable

technique(s)

blocking: suitable, cell culture | mammalian: suitable, hybridization: suitable, protein purification: suitable, transformation: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

DNA extraction: suitable, RNA extraction: suitable, cell culture | mammalian: suitable, protein purification: suitable

impurities

≤3.0% water

impurities

≤0.05% Phosphorus (P)

impurities

-

impurities

-

CMC

0.06 mM (20-25°C)

CMC

0.06 mM (20-25°C)

CMC

0.06 mM (20-25°C)

CMC

-

General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.

Application

TWEEN 20 has been used as a component of:
  • washing buffer and blocking buffer in Western blotting
  • blocking buffer in immunohistochemistry
  • washing buffer in ELISA (enzyme linked immunosorbent assay)
  • reaction mixture in PCR (polymerase chain reaction)
TWEEN 20 is a nonionic detergent widely used in biochemical applications. It has been used as an emulsifying agent for the preparation of stable oil-in-water emulsions. TWEEN 20 has been used in pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins).
TWEEN 20 has been used as a blocking agent for membrane based immunoassays at a typical concentration of 0.05%. TWEEN 20 can be used for lysing mammalian cells at a concentration of 0.005 to 0.5%.
Non-ionic detergent

Packaging

P1379-1KT:
Each kit contains 3 x 100ML samples, each sample from a uniquely manufactured lot.

Preparation Note

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

527.0 °F - Pensky-Martens closed cup

Flash Point(C)

275 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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