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P9416

Sigma-Aldrich

TWEEN® 20

for molecular biology, viscous liquid

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Synonym(s):
Polyethylene glycol sorbitan monolaurate, Polyoxyethylenesorbitan monolaurate
CAS Number:
MDL number:
NACRES:
NA.31

biological source

corn
plant (Palm)
plant (coconut)
wheat

grade

for molecular biology

description

non-ionic

Assay

>40% (GC)

form

viscous liquid

mol wt

~1228
~1228

composition

lauric acid, ≥40% (balance primarily myristic, palmitic, and stearic acids)

concentration

≥40.0% (GC)

impurities

RNAse, none detected
endonuclease, none detected
exonuclease, none detected

refractive index

n20/D 1.468 (lit.)

CMC

60 mg/L

solubility

water: 100 mg/mL, clear, colorless to yellow

density

1.095 g/mL at 25 °C (lit.)

suitability

suitable for molecular biology

foreign activity

Endonuclease-exonuclease and RNase, none detected

InChI

1S/C26H50O10/c1-2-3-4-5-6-7-8-9-10-11-24(30)34-19-18-31-20-22(32-15-12-27)26-25(35-17-14-29)23(21-36-26)33-16-13-28/h22-23,25-29H,2-21H2,1H3

InChI key

HMFKFHLTUCJZJO-UHFFFAOYSA-N

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This Item
P2287P1379P8341
TWEEN® 20 for molecular biology, viscous liquid

Sigma-Aldrich

P9416

TWEEN® 20

TWEEN® 20 viscous liquid, suitable for cell culture

Sigma-Aldrich

P2287

TWEEN® 20

TWEEN® 20 viscous liquid

Sigma-Aldrich

P1379

TWEEN® 20

TWEEN® 20 Low-peroxide; Low-carbonyls

Sigma-Aldrich

P8341

TWEEN® 20

grade

for molecular biology

grade

-

grade

-

grade

-

description

non-ionic

description

non-ionic

description

non-ionic

description

non-ionic

assay

>40% (GC)

assay

-

assay

>40% (GC)

assay

-

form

viscous liquid

form

viscous liquid

form

viscous liquid

form

viscous liquid

mol wt

~1228

mol wt

~1228

mol wt

~1228

mol wt

~1228

General description

Tween®20 is a polyoxyethylene sorbitol ester that belongs to the polysorbate family. It is a nonionic detergent having a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, 1 sorbitol, and 1 lauric acid as the primary fatty acid. The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Sorbitol forms the backbone ring to which the ethylene oxide polymers are attached.
Tween® 20 is a nonionic surfactant and detergent widely applied in molecular biology, biochemical, and life science research. Its predominant use involves the solubilization of membrane proteins during the isolation of membrane-protein complexes. Additionally, it serves as a component in various washing, blocking, dilution, and stabilization buffers. Additionally, it functions as a reagent for immunohistochemistry, encompassing techniques like Western blotting and ELISAs. In these applications, Tween 20 plays crucial roles such as preventing non-specific antibody binding, minimizing background staining, and ensuring the even distribution of reagents.

Surfactants, possessing both hydrophilic and hydrophobic components, fall into two main categories: ionic, which ionize in water (anionic, cationic, or ampholytic), and nonionic, which do not ionize. The discussed product is a nonionic surfactant with diverse applications such as emulsification, dispersion, solubilization, and detergent use. Additionally, it can serve as a solubilizer for membrane proteins.

Application

TWEEN 20 is widely used in biochemical applications. It has been used:
  • As an emulsifying agent for the preparation of stable oil-in-water emulsions
  • In pre-extraction of membranes to remove peripheral proteins (used at 2% for extraction of membrane-bound proteins)
  • As a blocking agent for membrane based immunoassays at a typical concentration of 0.05%
  • For lysing mammalian cells at a concentration of 0.005 to 0.5%
  • Along with PBS in the dilution of antibodies in immunohistochemistry technique
  • For washing cells in FISH (Fluorescence in situ hybridization) technique
Non-ionic detergent

Features and Benefits

  • Highly versatile surfactant for your Life Science and Biochemical research
  • Suitable for sensitive molecular biology research applications
  • Tested for Endonuclease, exonuclease and Rnase′s
  • Excellent solubilising, dispersing and emulsifying agent

Caution

TWEEN 20 may not be stable to autoclaving, particularly with metal cations in buffer solutions. Autoclaving is not recommended without testing for changes in properties. TWEEN 20 is heat sensitive and will darken when exposed to elevated temperatures. Polysorbates have been reported to be incompatible with alkalis, heavy metal salts, phenols, and tannic acid. They may also reduce the activity of many preservatives. Aqueous solutions of polysorbates undergo autoxidation during storage, with changes being catalyzed by light, increased temperature, and copper sulfate.

Preparation Note

TWEEN 20 is miscible in water (100mg/mL), yielding a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate. TWEEN 20 is insoluble in liquid paraffin and fixed oils.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

527.0 °F - Pensky-Martens closed cup

Flash Point(C)

275 °C - Pensky-Martens closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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