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P4417

Sigma-Aldrich

Phosphate buffered saline

tablet

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Synonym(s):
PBS
MDL number:
eCl@ss:
32129211
NACRES:
NA.25

form

tablet

Quality Level

pH

7.2-7.6 (1 tablet/200 mL)

solubility

water: soluble

application(s)

diagnostic assay manufacturing

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This Item
P5119P3813P3688
pH

7.2-7.6 (1 tablet/200 mL)

pH

7.2 (25 °C)

pH

7.4

pH

7.4

solubility

water: soluble

solubility

-

solubility

water: soluble

solubility

water: soluble

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Phosphate-buffered saline (PBS) is a widely utilized buffer solution in biological research, valued for its versatility and essential attributes. Notably, its isotonicity closely mirrors the pH, osmolarity, and ion concentrations of the human body, rendering it non-toxic to cells. This pivotal characteristic plays a crucial role in diverse cell biology procedures, including the gentle washing of cells, safe transportation of tissues, and precise dilution of cells and reagents. These applications contribute to the preservation of cell viability and the establishment of a conducive physiological environment. The buffering capacity of PBS, sustained by a phosphate-phosphoric acid equilibrium, establishes a stable pH environment crucial for optimal enzyme activity and various biological processes. This makes PBS an ideal choice for tasks such as preparing reagents, protein dialysis, and immunohistochemistry, ensuring the reproducibility and reliability of experimental results.

Expanding beyond cell biology, PBS demonstrates versatility in applications such as creating custom ELISA buffers for optimal antibody-antigen interactions. Moreover, it serves as a proficient running buffer for Protein A/G chromatography, facilitating antibody purification based on specific antigen binding. In hematological and biochemical procedures, PBS plays a pivotal role in the effective washing of cells and tissues. In summary, PBS stands out as an indispensable tool in both biological and biochemical research, offering isotonicity, buffering capacity, simplicity, and stability. Its diverse applications in cell biology, reagent preparation, protein purification, and various biological assays underscore its profound significance in advancing scientific knowledge and understanding.

Application

Phosphate buffered saline has been used-
  • for the production of platinum nanoparticles (NP-Pt) hydrocolloids
  • for the washing of blood sample and SEM (scanning electron microscope) stubs prior to SEM
  • for preparing the solution of fluorescent lectin wheat germ agglutinin (WGA)
  • for the dilution of capture antibody during ELISA (enzyme linked immunosorbent assay)
  • for suspension of cell pellets during centrifugation
  • in immunoassay procedures

Features and Benefits

  • High-quality product suitable for multiple research applications
  • Can be used as a buffer component in biological and biochemical applications
  • Highly soluble in water

Reconstitution

One tablet dissolved in 200 mL of deionized water yields 0.01 M phosphate buffer, 0.0027 M potassium chloride and 0.137 M sodium chloride, pH 7.4, at 25 °C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Sputum cathelicidin, urokinase plasminogen activation system components, and cytokines discriminate cystic fibrosis, COPD, and asthma inflammation.
Xiao W et al
Chest, 128(4), 2316-2326 (2005)
Structural damage of chicken red blood cells exposed to platinum nanoparticles and cisplatin.
Kutwin M et al
Nanoscale Research Letters, 9(1), 257-257 (2014)
Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins.
Lopez C et al
Food Chemistry, 120.1, 22-33 (2010)
M Michaelis et al.
The journal of physical chemistry. B, 123(31), 6694-6704 (2019-07-11)
Although successful in the structural determination of ordered biomolecules, the spectroscopic investigation of oligopeptides in solution is hindered by their complex and rapidly changing conformational ensemble. The measured circular dichroism (CD) spectrum of an oligopeptide is an ensemble average over
Simon C Drew et al.
PloS one, 5(12), e15875-e15875 (2011-01-07)
The amyloid-β (Aβ) peptide is the primary component of the extracellular senile plaques characteristic of Alzheimer's disease (AD). The metals hypothesis implicates redox-active copper ions in the pathogenesis of AD and the Cu(2+) coordination of various Aβ peptides has been

Protocols

Antibody Purification using Protein A, Protein G, or Protein L Agarose

Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum or ascites the final preparation will contain endogenous host IgG as well as specific antibodies. In general, the presence of this endogenous IgG should not interfere with assays using the antibodies.

Immunofluorescence Labeling of Cells

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

ELISA Procedures

ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

Immunoprecipitation Procedure

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

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