C9858

Creatine Kinase MM Fraction from human heart

lyophilized powder

• Synonym(s): CK-MM Fraction, Human CK MM, Human Creatine Kinase
• MDL number: MFCD00151687
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥100 units/mg protein
• UniProt accession no.: P06732
• storage temp.: −20°C
• Gene Information: human ... CKM(1158)

Description

Specificity

One unit will transfer 1.0 μmole of phosphate from creatine phosphate to ADP per minute at 37 °C (measured at 340 nm as one equimolar amount of NADH produced by coupled reaction).

Application

Creatine kinase MM fraction from human heart has been used in a study to investigate the importance of intraoperative and postoperative cardiac medical therapy in emergency coronary artery bypass grafting for acute myocardial infarction. Creatine kinase MM fraction from human heart has also been used in a study to investigate the circadian dependence of infarct size and left ventricular function after ST slevation myocardial infarction.

Biochem/physiol Actions

May be used as a control or calibrator in monitoring myocardial injury.

Warning

All human source materials have tested negative for HIV1, HIV2, HCV antibodies and HbsAg. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious.

Unit Definition

One unit will transfer one micromole of phosphate from creatine phosphate to ADP per minute at 37 deg C.

Physical form

Lyophilized powder containing Tris-HCl, EDTA and N-acetyl cysteine.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable