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product name
A2H, 85011441
biological source
hamster ovary
growth mode
Adherent
karyotype
2n = 22
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Hamster Chinese ovary
Cell Line Description
Used for the induction and isolation of nutritionally deficient mutants. A reclone of ′Clone A′ established by Puck (the ′H′ denotes MRC Harwell). Useful for genetic studies due to the low chromosome number.
Application
Genetic studies
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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