Recommended Products
product name
BC3H1, 86093001
biological source
mouse brain
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast
products
Acetylcholine
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse brain tumour
Cell Line Description
Established in 1974 from a mouse brain neoplasm tumour induced by nitrosoethylurea, possess many properties characteristic of smooth muscle. Since then it has been suggested that BC3H1 is a skeletal line of ectodermal origin that is defective for commitment to terminal differentiation. The two distinct morphologies typical of this cell line are large spheroid cells and smooth-muscle-like cells. The fusiform is the more common.
Application
Acetylcholine receptor studies.
Culture Medium
DMEM + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Subculture Routine
Spilt confluent cultures 1:3 to 1:10 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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