D0530

Dialysis tubing, high retention seamless cellulose tubing

avg. flat width 32 mm (1.3 in.), MWCO 12400, 99.99% retention

• Synonym(s): Dialysis tubing
• UNSPSC Code: 41123100
• NACRES: NB.51

Properties

• avg. diam.: 20 mm , when full
• avg. flat width: 32 mm (1.3 in.)
• capacity: ~100 mL/ft
• pore size: 12400 MWCO, 99.99% retention

Description

General description

Retains >99% of cytochrome c (M.W. 12,400) in solution over a 10-hour period.

Packaging

Supplied in rolls, dry

Quality

May contain glycerin and sulfur compounds in more than trace amounts; washing instructions are included.

Preparation Note

Removal of glycerol included as a humectant can be accomplished by washing the tubing in running water for 3-4 hours. Removal of sulfur compounds can be accomplished by treating the tubing with a 0.3% (w/v) solution of sodium sulfide at 80°C for 1 minute. Wash with hot water (60°C) for 2 minutes, followed by acidification with a 0.2% (v/v) solution of sulfuric acid, then rinse with hot water to remove the acid. This tubing will retain most proteins of molecular weight 12,000 or greater.