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GE17-0948-01

Blue Sepharose 6 Fast Flow

Cytiva 17-0948-01, pack of 50 mL

Synonym(s):

Blue Sepharose Fast Flow

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

Cibacron Blue

packaging

pack of 50 mL

manufacturer/tradename

Cytiva 17-0948-01

storage condition

(20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0)

matrix

6% cross-linked agarose

particle size

45-165 μm

average diameter

90 μm

cleaning in place

3-13

working range

4-12

suitability

suitable for bioprocess medium

storage temp.

2-8°C

General description

Blue Sepharose 6 Fast Flow is Cibacron Blue 3G coupled to Sepharose 6 Fast Flow. This medium is well established as adsorbent for albumin and interferon at both laboratory and process scale.

Blue Sepharose 6 Fast Flow is a member of the Cytiva range of affinity chromatography media for capture and intermediate purification.
Blue Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with Cibacron Blue 3G covalently attached by the triazine coupling method.
The blue dye binds many proteins, such as albumin, interferon, lipoproteins and blood coagulation factors. It also binds several enzymes including kinases, dehydrogenases, and most enzymes requiring adenyl-containing cofactors e.g. NAD+.

Blue Sepharose 6 Fast Flow has been developed and tested in co-operation with leading large scale manufacturers and is used in routine commercial production.

Blue Sepharose 6 Fast Flow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development.
As member of the BioProcess media range, Blue Sepharose 6 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Designed for large-scale purification of interferon and albumin. Also used for isolating groups of enzymes and removing of albumin
  • BioProcess medium supported for industrial applications and well-established in approved processes
  • Cibacron Blue 3G covalently coupled to the base matrix allowing for low ligand leakage and high capacity

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Storage Class Code

3 - Flammable liquids


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

This page shows how to convert between flow velocity and volumetric flow rate in affinity chromatography of antibodies.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocols

This page shows how to separate or remove albumin by affinity chromatography using HiTrap Blue HP and Blue Sepharose 6 Fast Flow.

Remove albumin from affinity chromatography samples using HiTrap™ Blue HP or Blue Sepharose® 6 Fast Flow from Cytiva.

Affinity chromatography purification of enzymes using 5’ AMP Sepharose® 4B, HiTrap® Blue HP, and Blue Sepharose® 6 Fast Flow products.

This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.

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