H9377

Hypoxanthine

≥99.0%

• Synonym(s): 6-Hydroxypurine
• Empirical Formula (Hill Notation): C₅H₄N₄O
• CAS Number: 68-94-0
• Molecular Weight: 136.11
• Beilstein: 5811
• EC Number: 200-697-3
• MDL number: MFCD00005725
• UNSPSC Code: 41106305
• PubChem Substance ID: 24895850
• NACRES: NA.51

Properties

• biological source: synthetic (organic)
• Quality Level: 300
• Assay: ≥99.0%
• form: powder
• mp: >300 °C (lit.)
• solubility: formic acid: water (2:1): 50 mg/mL, clear to slightly hazy, colorless to yellow
• SMILES string: O=C1NC=Nc2nc[nH]c12
• InChI: 1S/C5H4N4O/c10-5-3-4(7-1-6-3)8-2-9-5/h1-2H,(H2,6,7,8,9,10)
• InChI key: FDGQSTZJBFJUBT-UHFFFAOYSA-N

Description

Application

Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.

Hypoxanthine has been used:

• to gavage mice and to evaluate the activity of xanthine oxidase (XO) in pasteurized whole milk in vivo in potassium oxonate (PO) -induced mouse model of hyperuricemia
• as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) medium to cultivate in-vitro cell line (EL-1 cells)
• as a pure standard for the quantification of hypoxanthine in vitreous fluid sample using high-performance liquid chromatography-ultra-violet (HPLC-UV) for the estimation of post-mortem interval

Biochem/physiol Actions

Hypoxanthine acts as an intermediate in purine metabolism. It elicits a vital role as a 5′-base of the anticodon in a few transfer-RNAs. Though similar to guanine (Gua), it is devoid of the exocyclic amino group at C(2) position. It may be used as a substrate to study the specificity and kinetics of hypoxanthine-guanine phosphoribosyl transferases.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves