M5563
Mimetic Red 3 Ligand Affinity Adsorbent
suspension
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Application
Ligand affinity adsorbents are used in protein chromatography, affinity chromatography and dye resins. Ligand affinity adsorbents have been used to describe a simple protocol for the purification of Taq Polymerase from E. coli lysates as well as for the purification of other DNA polymerases.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
95.0 °F - closed cup
Flash Point(C)
35 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Sotirios Melissis et al.
Biotechnology journal, 2(1), 121-132 (2006-12-22)
The thermostable Thermus aquaticus DNA polymerase (Taq Pol) has been the key factor in transforming the initial PCR method into one with huge impact in molecular biology and biotechnology. Therefore, the development of effective affinity adsorbents for the purification of
Jeannie Horak et al.
Analytical and bioanalytical chemistry, 400(8), 2349-2359 (2010-12-22)
The new affinity-type Mimetic Ligand™ B14 was coupled with a 1,2-diaminoethane spacer (2LP) and a [1,2,3]-triazole spacer (TRZ) to three different support media. In addition to the agarose-based PuraBead and the polymethacrylate-type Fractogel, three new polymeric support media were introduced
S Melissis et al.
Journal of chromatography. A, 1122(1-2), 63-75 (2006-05-23)
The commercial availability of DNA polymerases has revolutionized molecular biotechnology and certain sectors of the bio-industry. Therefore, the development of affinity adsorbents for purification of DNA polymerases is of academic interest and practical importance. In the present study we describe
C R Lowe et al.
Trends in biotechnology, 10(12), 442-448 (1992-12-01)
Affinity chromatography has been extensively refined over the past few years to meet the more stringent criteria being placed on recombinant proteins as therapeutic products. New developments in the design of selective and stable ligands for affinity chromatography are establishing
S J Burton
Methods in molecular biology (Clifton, N.J.), 11, 91-103 (1992-01-01)
Dye-ligand affinity chromatography is performed with an adsorbent consisting of a solid support matrix to which a dye has been cova-lently bonded. The protein mixture is passed through a packed bed of adsorbent that selectively binds proteins that are able
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