MAK499
Glutamate Dehydrogenase (GDH) Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
Assay Kit for Glutamate Dehydrogenase Activity
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About This Item
UNSPSC Code:
12352204
NACRES:
NA.84
Recommended Products
Quality Level
application(s)
agriculture
detection method
colorimetric
relevant disease(s)
cancer; gastrointestinal diseases
shipped in
wet ice
storage temp.
−20°C
General description
Glutamate dehydrogenase (GLDH) is an enzyme which catalyzes the interconversion of glutamate and α-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the study of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections.
Features and Benefits
- Linear detection range: 0.4 to 80 U/L for 30 minute reaction. Detection Limit of 0.1 U/L for 120 minute reaction.
- Compatible with high-throughput handling systems.
Suitability
The kit is suitable for glutamate dehydrogenase activity determination in biological samples (e.g. plasma, serum, urine, tissue, and culture media.)
Principle
The non-radioactive, colorimetric Glutamate Dehydrogenase (GDH) Assay Kit is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
3 - Flammable liquids
WGK
WGK 2
Certificates of Analysis (COA)
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