MAK501
Glucose Oxidase Activity Assay Kit
sufficient for 100 colorimetric or fluorometric tests
Synonym(s):
Glucose Oxidase Assay Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
Quality Level
application(s)
food and beverages
pharmaceutical
detection method
colorimetric
fluorometric
relevant disease(s)
endocrinological disorders, diabetes
shipped in
wet ice
storage temp.
−20°C
General description
Glucose oxidase catalyzes the oxidation of glucose from D-glucose to D-glucono-δ-lactone. Physiologically, it aids in the breakdown of glucose into smaller metabolites. It is widely used in electrochemical glucose sensors designed for diabetes patients.
Features and Benefits
- Linear detection range: 0.02 to 10 U/L glucose oxidase for the colorimetric assay and 0.002 to 1.5 U/L for the fluorometric assay.
Suitability
The kit is suitable for determining glucose oxidase activity in cell lysate, culture medium and other biological samples, as well as for studying the effects of drugs on glucose metabolism.
Principle
The Glucose Oxidase Assay Kit uses a single working reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570 nm or fluorescence intensity at λEx = 530 nm/λEm = 585 nm is directly proportional to glucose oxidase activity in the sample.
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Certificates of Analysis (COA)
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Protocols
Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.
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