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MAK561

Sigma-Aldrich

Succinate Dehydrogenase Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

SDH Activity Assay Kit

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About This Item

UNSPSC Code:
41116104
NACRES:
NA.28

usage

sufficient for 100 colorimetric tests

Quality Level

100

detection method

colorimetric

relevant disease(s)

neurological disorders

storage temp.

−20°C

General description

Succinate Dehydrogenase (SDH) is part of complex II found in the inner part of the mitochondrial membrane. SDH is part of the citric acid cycle (TCA) that catalyze the oxidation of succinate to fumarate. Electrons from succinate are transferred to FAD forming FADH2 which transfer electrons to CoQ. Mutations in SDH cause hereditary paraganglioma/phaeochromocytoma syndrome and a neurodegenerative disorder known as Leigh syndrome. The Succinate Dehydrogenase Assay kit provides a simple and sensitive procedure for measuring SDH activity in a variety of tissues, cells, and isolated mitochondria. SDH activity is determined by generating a product with absorbance at 600 nm proportional to the enzymatic activity present. One unit of SDH is the amount of enzyme that generates 1.0 μmole of DCIP per minute at pH 7.2 at 25 °C.

Application

Succinate Dehydrogenase Assay Kit has been used to measure the SDH enzyme activity in cell and tissue culture supernatants and in purified mitchondria.

Features and Benefits

  • Highly sensitive, simple and high-throughput assay for measuring Succinate Dehydrogenase (SDH) concentration.
  • Supportive calculator will calculate the reagents needed according to your experimental needs, and analyze your results based on your experimental data.
Click here to download a calculator excel file

Suitability

Suitable for the detection of Succinate Dehydrogenase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

Pictograms

Skull and crossbonesEnvironment
GHS06,GHS09

Signal Word

Danger

Hazard Statements

H302,H311 + H331,H411

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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