MBD0055
MediaBoost
non-animal sterile microbial growth supplement
Synonym(s):
growth supplement for germination, microbial culture additive, microbial culture enhancer, microbial media supplement, minimal growth media supplement
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About This Item
UNSPSC Code:
41106212
NACRES:
NB.21
Recommended Products
Quality Level
sterility
sterile
form
liquid
technique(s)
microbiological culture: suitable
shipped in
wet ice
storage temp.
2-8°C
suitability
Bacillus spp.
Escherichia spp.
Lactobacillus spp.
Lactococcus spp.
Pseudomonas spp.
Saccharomyces spp.
bacteria
yeasts
General description
Studying microbial species via cutting edge genomic techniques such as next generation sequencing (NGS) has become increasingly prevalent in recent years; however, genomic methods alone cannot fully replace experimental testing of microbial species. Therefore, the ability to culture microbes in a laboratory setting remains essential for comprehensive study of these communities. Some microbial species can be challenging to culture in standard culture media. Often species of interest or a specific process require specialized media compositions, to provide an optimal environment for maximal proliferation and yield. A few examples includes
- Chemically defined minimal growth media are often used to grow bacteria for proteomics, as a base for stable isotope enrichment in NMR protein structure-function studies, but growth can be limited without the addition of an effective supplement.
- Use of chemically defined minimal growth media in the production of secreted microbial proteins to ensure reproducibility and a higher concentration of the microbial-produced protein of interest.
Application
MediaBoost provides microbial researchers a ready-to-use media supplement to amplify growth in selected gram-positive bacteria, gram-negative bacteria, and yeast species. Application data is available for some of the strains and media combinations that have been tested with MediaBoost, including:
Please note strains and media combinations and should be tested on a case-by-case basis by the researcher for experiment optimization.
- Bacillus subtilis spores in LB medium
- Lactobacillus rhamnosus in LB medium
- Pseudomonas aeruginosa in M9 medium
- Saccharomyces cerevisiae in YPD, BM2, and IMDM media
Please note strains and media combinations and should be tested on a case-by-case basis by the researcher for experiment optimization.
Features and Benefits
- Boosts Growth of Certain Microorganisms
- Reduces Germination Time
- Ready-Made, Sterile Solution
- Protein-Free
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Anna Gąciarz et al.
Microbial cell factories, 16(1), 108-108 (2017-06-18)
The production of recombinant proteins containing disulfide bonds in Escherichia coli is challenging. In most cases the protein of interest needs to be either targeted to the oxidizing periplasm or expressed in the cytoplasm in the form of inclusion bodies
M Bonnet et al.
New microbes and new infections, 34, 100622-100622 (2020-01-21)
Microbiology has been largely developed thanks to the discovery and optimization of culture media. The first liquid artificial culture medium was created by Louis Pasteur in 1860. Previously, bacterial growth on daily materials such as some foods had been observed.
Stephan B Azatian et al.
Journal of biomolecular NMR, 73(1-2), 11-17 (2019-01-08)
We describe a general and simple modification to the standard M9 minimal medium recipe that leads to an approximate twofold increase in the yield of heterologously expressed proteins in Escherichia coli BL21(DE3) bacteria. We monitored the growth of bacteria transformed
Maryam Tidjani Alou et al.
Clinical microbiology reviews, 34(1) (2020-10-30)
The last 5 years have seen a turning point in the study of the gut microbiota with a rebirth of culture-dependent approaches to study the gut microbiota. High-throughput methods have been developed to study bacterial diversity with culture conditions aimed
William H Lewis et al.
Nature reviews. Microbiology, 19(4), 225-240 (2020-10-24)
Despite the surge of microbial genome data, experimental testing is important to confirm inferences about the cell biology, ecological roles and evolution of microorganisms. As the majority of archaeal and bacterial diversity remains uncultured and poorly characterized, culturing is a
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