RAB0408
Mouse Prolactin ELISA Kit
for serum, plasma and cell culture supernatant
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About This Item
UNSPSC Code:
41116158
NACRES:
NA.84
Recommended Products
species reactivity
mouse
packaging
kit of 96 wells (12 strips x 8 wells)
technique(s)
ELISA: suitable
capture ELISA: suitable
input
sample type plasma
sample type serum
sample type cell culture supernatant(s)
assay range
inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 30 pg/mL
standard curve range: 27.43-20000 pg/mL
detection method
colorimetric
shipped in
wet ice
storage temp.
−20°C
Gene Information
mouse ... Prl(19109)
General description
The Mouse Prolactin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Prolactin in serum, plasma, cell culture supernatants and urine.
Immunogen
Recombinant Mouse Prolactin
Application
For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)
Biochem/physiol Actions
Prolactin (Prl) is processed to lower molecular weight N-terminal fragments, 14-18kDa, called vasoinhibins, which have antiangiogenic effects. In endothelial cells, the 16kDa prolactin form exerts antiproliferative and proapoptotic effects which result in cell cycle arrest, caspase and NFκB (nuclear factor) activation and regulation of Bcl-2 protein family. In sexually mature female mice, the plasma levels of prolactin remain constant throughout estrous cycle, but show a sudden increase during proestrous cycle due to increase in circulating levels of estrogens. Studies show that Prl activates T cells, B cells, natural killer (NK) cells, macrophages, neutrophils and dendritic cells and there is a significant level of hyperprolactinemia incidence in patients with autoimmune diseases, such as rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). Studies in rats show that Prl is involved in T cell homing to mammary glands during early lactation and that hypoprolactemia during lactation results in modified T cell distribution with increased T cell in circulation and reduced in mammary glands.
Other Notes
A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.
Please type the word sample in the text box provided for lot number.
Kit Components Also Available Separately
Product No.
Description
SDS
- RABELADAELISA 1X Assay/Sample Diluent Buffer A (Item D1)SDS
- RABELADBELISA 5X Assay/Sample Diluent Buffer B (Item E1)SDS
- RABELADCELISA 1X Assay/Sample Diluent Buffer C (Item L)SDS
- RABSTOP3ELISA Stop Solution (Item I)SDS
- RABTMB3ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)SDS
- RABWASH420X Wash Buffer (Item B)SDS
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Met. Corr. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Impaired mammary gland T cell population during early lactation in hypoprolactinemic lactation-deficient rats.
Mackern-Oberti JP
Reproduction (Cambridge, England) null
N-terminal prolactin-derived fragments, vasoinhibins, are proapoptoptic and antiproliferative in the anterior pituitary.
Ferraris J
PLoS ONE null
Conditional Deletion of the Prolactin Receptor to Identify Brain-Specific Actions of Prolactin
3 Biotech null
Daniella C Buonfiglio et al.
Hormones and behavior, 71, 60-68 (2015-04-22)
Prolactin and placental lactogens control mammary development and lactation as well as play an important role in maternal behaviors. However, the molecular mechanisms in the brain responsible for this regulation remain largely unknown. Therefore, the present study investigated whether Signal
John D Landua et al.
Journal of mammary gland biology and neoplasia, 25(2), 145-162 (2020-07-25)
Targeted disruption of the murine Hoxd10 gene (ΔHoxd10) leads to a high frequency of localized (gland-to-gland or regionally within a gland) lactation impairment in homozygous mutant mice as a single gene mutation. The effect of Hoxd10 disruption was enhanced by
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